Mouse skin as an in vitro model to predict the transdermal absorption of moxidectin in cattle

SALLOVITZ, J.; LIFSCHITZ, A.; IMPERIALE, F.; VIRKEL, G.; LANUSSE, C.

Córdoba, Argentina

Congreso; XXXVIII Reunión Anual de la Sociedad Argentina de Farmacología Experimental; 2006

Sociedad Argentina de Farmacología Experimental

MOUSE SKIN AS AN IN VITRO MODEL TO PREDICT THE TRANSDERMAL ABSORPTION OF MOXIDECTIN IN CATTLE. Sallovitz, J.(1,2), Lifschitz, A.(2,3), Imperiale, F. (2,3), Virkel, G., Lanusse, C. (2,3). (1) Lab. de Farmacología, Dpto. Fisiopatología, FCV-UNCPBA, 7000-Tandil; (2) CICPBA.; (3) CONICET, Argentina. E-mail: juan@vet.unicen.edu.ar Endectocide parasitic compounds are extensively used for broad-spectrum parasite control and their topical administration to cattle is widespread in clinical practice. However, there is not a practical, rapid and easy method to determine the absorption of new topical formulations before clinical trials. The objective of the present work was to characterize the transdermal absorption of two topical formulation of moxidectin (MXD) using mouse skin as an in vitro model. Subcutaneous tissues were rubbed using an ethanol-wet cotton. Mouse skins were mounted in Franz-type diffusion cells and treated with two commercial formulations (1.7 mL) for use in cattle. The receptor medium was buffer phosphate (0.1 M), bovine albumin (4.5%) and ethanol (20%). MXD concentrations in the receptor medium were measured by HPLC with a fluorescence detector. MXD percutaneous absorption reached a steady state at 24 hours post-administration. There were no statistical differences between the penetration rates of both formulations (18.1 ± 11.4 vs. 22 ± 13.6 ng.cm-2.h-1, P>0.05). The correlation coefficient (r) between penetration rates and plasma AUCpartial from previous in vivo works was 0.9329 (CI 95%: 0.6046 to 0.9903). This investigation contributes to the searching for an in vitro model to predict the percutaneous absorption of topical formulation of endectocides in cattle. MODALIDAD DE PRESENTACIÓN: POSTER