INVESTIGADORES
PARISI Gustavo Daniel
artículos
Título:
The tight-adhesion protein TadG of Bradyrhizobium diazoefficiens USDA 110 is involved in cell adhesion and infectivity on soybean roots.
Autor/es:
ELÍAS J. MONGIARDINI,; GUSTAVO PARISI; IGNACIO QUELAS; ANIBAL LODEIRO
Revista:
MICROBIOLOGICAL RESEARCH
Editorial:
ELSEVIER GMBH
Referencias:
Año: 2016
ISSN:
0944-5013
Resumen:
Adhesion of symbiotic bacteria to host plants is an essential early step of the infection process thatleads to the beneficial interaction. In the Bradyrhizobium diazoefficiens-soybean symbiosis few moleculardeterminants of adhesion are known. Here we identified the tight-adhesion gene products TadGEF inthe open-reading frames blr3941?blr3943 of the B. diazoefficiens USDA 110 complete genomic sequence.Predicted structure of TadG indicates a transmembrane domain and two extracytosolic domains, fromwhich the C-terminal has an integrin fold. TadE and TadF are also predicted as bearing transmembranesegments. Mutants in tadG or the small cluster tadGEF were impaired in adhesion to soybean roots,and the root infection was delayed. However, nodule histology was not compromised by the mutations,indicating that these effects were restricted to the earliest contact of the B. diazoefficiens and root surfaces.Knowledge of preinfection determinants is important for development of inoculants that are applied tosoybean crops worldwide.
