Phenotypic and functional changes on phagocytic cells recruited at the site of Candida albicans infection after chronic varied stress exposure

RODRIGUEZ GALAN MC; CORREA SG; IRIBARREN P; SOTOMAYOR CE

MEDICAL MYCOLOGY

TAYLOR & FRANCIS LTD

Año: 2002 vol. 40 p. 485 - 492

1369-3786

The transition of Candida albicans from commensalism to pathogenicity isassociated with the immune status of the host; resistance to fungus involvesmacrophages (Mf) and polymorphonuclear neutrophils (PMN), which act aseffector cells. T-cell function is also involved. Previously, we found that in Wistarrats exposed to chronic varied stress (CVS) immediately after C. albicans infection(Ca-S group) some functions of phagocytic cells, such as killer activity and NOproduction, were strongly modiŽed compared with unstressed, infected animals (Cagroup). We examined the phenotypic and functional changes of these effector cellsrecruited at the site of C. albicans infection. The recruitment of peritoneal cells (PC)was markedly reduced in Ca-S animals and the arrival of Mf and PMN was selectively diminished after CVS exposure. The integrin CD11b/CD18, implicated in migration and C. albicans phagocytosis, was downregulated in Mf of Ca-S animals. The activation markers CD54 and MHC-II were upregulated in Mf after fungal contact. The expression of CD54 was only changed in Ca-S rats. Finally, TNF-a production was reduced in PC of Ca-S animals, suggesting an impairment of functional activity. Taken together, the phenotypic and functional changes detectedin effector cells may account for the decreased resistance to candidiasis seen in conjunction with CVS. The changes seen also expand our knowledge of the role of Mf in the control of C. albicans dissemination.