Antitumoral effects of Pleurotus Ostreatus I-fraction in breast cancer.

RAMBURGER,R; FERRONATO, MJ; GUEVARA, JA; CUESTAS, JM; POTEMSKY, PD; FACCHINETTI, MM; CURINO,.AC; ALONSO, EN

Mar del Plata

Congreso; REUNIÓN ANUAL DE SOCIEDADES DE BIOCIENCIA 2019; 2019

Sociedad Argentina de Investigación Clínica

Mushrooms are considered as "small pharmaceutical factories" producinghundreds of bioactive compounds, many of which have shown to exertantitumoral activity in different types of cancer. Argentina has a high mushrooms diversity and important scientific-technological development applied to itscultivation. However, the antitumoral phytotherapeutic potential of ediblemushrooms cultivated in our country has not yet been considered. In this context, the purpose of the current study is to determine the antitumoral activity in breastcancer of Pleurotus ostreatus I-Fraction, an extract of water-solublepolysaccharides obtained from fruiting body, initially evaluating its potentialimmuno-independent antitumoral activity. To achieve the proposed objective, weemployed a murine mammary adenocarcinoma 4T1 cells line and performed cellviability assays by colorimetric assay with crystal violet, cell cycle analysis by flow cytometry, and wound healing assay. We found that P. Ostreatus I-Fraction at concentration from 2.5 mg/mL and ranging from 1,0 to 2,5 mg/mL decreased theviability of 4T1 cells in a concentration-dependent manner, at 24 hours and 48hours respectively (p