Effect of pharmacologic and genetic p300 modulation in the progression of hormone-independent breast cancer.

FERMENTO ME,; VILLEGAS ME; GANDINI NA; ALONSO EA ; FERRONATO MJ; FACCHINETTI MM ; CURINO AC

Mar del Plata

Congreso; LXI REUNIÓN ANUAL DE LA SOCIEDAD ARGENTINA DE INVESTIGACIÓN CLÍNICA (SAIC); 2016

Sociedad argentina de investigación clínica (SAIC)

Breast cancer (BC) is a heterogeneous disease consisting of many subtypes that have different responses to treatment and different clinical outcomes, suggesting the need to find new molecular markers. We previously demonstrated that pharmacological inhibition of p300 acetylase function has an antitumoral role in murine hormone-independent (HI) BC LM3 cell line, and decreases tumor progression in a syngeneic murine model of LM3. As these results were obtained in a murine line, we decided to investigate the role p300 has on BC by performing pharmacological inhibition of p300 acetyl-transferase function and analyzing the effects on cell count, migration, invasion and adhesion in human triple negative BC (TNBC) MDA-MB-231 cell line and on tumor progression in a xenograft murine model of MDA-MB-231. We observed that inhibition of p300 reduced viability (WST and cell count), migration (wound healing assay), invasion (Matrigel) and adhesion (cell count) in MDA-MB-231 cells (p