INVESTIGADORES
CHIAPELLO Laura Silvina
artículos
Título:
Apoptosis induction by glucuronoxylomannan of Cryptococcus neoformans
Autor/es:
L. S. CHIAPELLO, M. P. AOKI, H. R. RUBINSTEIN & D. T. MASIH
Revista:
MEDICAL MYCOLOGY
Editorial:
Taylor and Francis
Referencias:
Año: 2003 vol. 41 p. 347 - 353
ISSN:
1369-3786
Resumen:
We studied the ability of glucuronoxylomannan (GXM), the major constituent of
Cryptococcus neoformans capsular polysaccharide, to induce apoptosis in lymphocytes
from normal rats. Spleen mononuclear cells (Smc) from normal rats treated
with GXM for 24 h exhibited, in comparison with controls, an increased
hypodiploidy in the DNA profile after staining with propidium iodide, as well as
increased ladder-type DNA fragmentation in agarose gel electrophoresis and a
high number of positive cells in the terminal deoxynucleotidyl transferase biotindUTP
nick end labeling (TUNEL) reaction. Furthermore, increased hypodiploidy
in the DNA profile was also observed in Smc expressing T-cell receptor (TCR/).
We also studied the induction of apoptosis in lungs and spleens from rats in the
immunosuppressor period of disseminated cryptococcosis. TUNEL labeling of
lungs and spleens from rats obtained 14 days after infection with C. neoformanscapsular polysaccharide, to induce apoptosis in lymphocytes
from normal rats. Spleen mononuclear cells (Smc) from normal rats treated
with GXM for 24 h exhibited, in comparison with controls, an increased
hypodiploidy in the DNA profile after staining with propidium iodide, as well as
increased ladder-type DNA fragmentation in agarose gel electrophoresis and a
high number of positive cells in the terminal deoxynucleotidyl transferase biotindUTP
nick end labeling (TUNEL) reaction. Furthermore, increased hypodiploidy
in the DNA profile was also observed in Smc expressing T-cell receptor (TCR/).
We also studied the induction of apoptosis in lungs and spleens from rats in the
immunosuppressor period of disseminated cryptococcosis. TUNEL labeling of
lungs and spleens from rats obtained 14 days after infection with C. neoformans/).
We also studied the induction of apoptosis in lungs and spleens from rats in the
immunosuppressor period of disseminated cryptococcosis. TUNEL labeling of
lungs and spleens from rats obtained 14 days after infection with C. neoformansC. neoformans
showed a large number of apoptotic cells. Our results provide strong cytometric,
molecular and morphological evidence that apoptosis could be a previously
unrecognized immunosuppressive property of GXM in vitro. This programmed cell
death may be involved in the immunosuppression observed during C. neoformansin vitro. This programmed cell
death may be involved in the immunosuppression observed during C. neoformansC. neoformans
infection.