INVESTIGADORES
CHIAPELLO Laura Silvina
artículos
Título:
Heat killed cells of Cryptococcus neoformans var. grubii
Autor/es:
JOSE´ L. BARONETTI, LAURA S. CHIAPELLO, MARI´A P. AOKI, SUSANA GEA & DIANA T. MASIH
Revista:
MEDICAL MYCOLOGY
Editorial:
Taylor and Francis
Referencias:
Año: 2006 vol. 44 p. 493 - 504
ISSN:
1369-3786
Resumen:
Different clinical parameters which included cell-mediated immune (CMI)
response, were evaluated in a model of disseminated cryptococcosis in rats. The
experimental animals were pretreated four days prior to their exposure to
Cryptococcus neoformans var. grubii with either heat killed cells of this yeastlike
pathogen (HKC) or capsular polysaccharide (CPS) emulsified in complete Freund
adjuvant (CFA). Rats treated with HKC-CFA and intraperitoneally infected withvar. grubii with either heat killed cells of this yeastlike
pathogen (HKC) or capsular polysaccharide (CPS) emulsified in complete Freund
adjuvant (CFA). Rats treated with HKC-CFA and intraperitoneally infected with
C. neoformans var. grubii had significantly better clearance of yeasts from tissues, a
lower concentration of the cryptococcal capsular polysaccharide, glucuronoxylomannan
(GXM), in serum and tissues, and better histopathological parameters
compared to unpretreated infected rats. In contrast, rats treated with CPS-CFA
presented an exacerbation of infection with a significantly higher fungal burden in
tissues, a higher concentration of GXM in serum, and worse histopathological
parameters compared to similar unpretreated infected rats. In addition, HKC-CFA
treatment produced a T helper 1 (Th1) profile with improvements in the spleen cell
proliferative response, in the level of INFg production by CD4 T cells, and in the
nitric oxide (NO) production by peritoneal cells. On the other hand, rats treated
with CPS-CFA showed an increased level of the immunoregulatory cytokine IL10
production by CD4 T cells, but no modification in the NO production by
peritoneal cells.var. grubii had significantly better clearance of yeasts from tissues, a
lower concentration of the cryptococcal capsular polysaccharide, glucuronoxylomannan
(GXM), in serum and tissues, and better histopathological parameters
compared to unpretreated infected rats. In contrast, rats treated with CPS-CFA
presented an exacerbation of infection with a significantly higher fungal burden in
tissues, a higher concentration of GXM in serum, and worse histopathological
parameters compared to similar unpretreated infected rats. In addition, HKC-CFA
treatment produced a T helper 1 (Th1) profile with improvements in the spleen cell
proliferative response, in the level of INFg production by CD4 T cells, and in the
nitric oxide (NO) production by peritoneal cells. On the other hand, rats treated
with CPS-CFA showed an increased level of the immunoregulatory cytokine IL10
production by CD4 T cells, but no modification in the NO production by
peritoneal cells.g production by CD4 T cells, and in the
nitric oxide (NO) production by peritoneal cells. On the other hand, rats treated
with CPS-CFA showed an increased level of the immunoregulatory cytokine IL10
production by CD4 T cells, but no modification in the NO production by
peritoneal cells.