Quantitation of cytokine gene expression by real time PCR in bovine milk and colostrum cells from cows immunized with a bovine rotavirus VP6 experimental vaccine.

DIEGO DANIEL GONZALEZ; AGUSTINA RIMONDI; MARIA SOL PEREZ-AGUIRREBURUALDE; MARINA MOZGOVOJ; DEMIAN BELLIDO; ANDRES WIGDOROVITZ; MARIA JOSE DUS SANTOS

RESEARCH IN VETERINARY SCIENCE

ELSEVIER SCI LTD

Lugar: Amsterdam; Año: 2013 p. 703 - 708

0034-5288

In a previous work, VP6 recombinant protein was produced using baculovirus system and it was evalu- 21 ated in a colostrum-deprived calf model. This vaccine was able to protect calves against viral challenge 22 without inducing neutralizing antibodies (NAb), suggesting that another immunological effectors were 23 involved in the protection observed. In this work, groups of cows (n = 4) were immunized in the last third 24 of gestation with a bovine rotavirus (BRV) experimental vaccine and with a VP6 subunit vaccine. At birth, 25 colostrums from vaccinated and non-vaccinated cows were processed and viable colostral mononuclear 26 cells were obtained. With the purpose of determining the cytokine patterns generated by cells from 27 immune secretions (colostrums and milk), a relative quantification by real time PCR was standardized. 28 Quantitative real time PCR (qPCR) was used to determine transcript levels of IL-4, IL-6, IL-10, IL-12, 29 IFN-c and IFN-a from these cells. Colostral and milk mononuclear cells expressed a different cytokine 30 transcript expression pattern regarding the vaccine used. These results demonstrated that the colostral 31 cellular population was active and could exert its action influencing the final immune response.