Enhanced enzymatic hydrolysis of xylan by Paenibacillaceae isolated from sugarcane bagasse

DÁVILA-COSTA MJ; VALDEÓN DH; RAGOUT AL; PEROTTI NI; ABATE CM; MARTÍNEZ MA

Cordoba, Argentina

Congreso; VI Congreso Argentino de la Sociedad de MIcrobiologia General (SAMIGE); 2009

Sociedad Argentina de Microbiologia General

In nature, cellulose and hemicelluloses, from which xylan is a major component, are associated with plant cell walls and are degraded by many microorganisms that synthesize a number of different enzymes in order to grow on them. From an industrial point of view, plant cell-wall degrading enzymes have become increasingly relevant in the hydrolysis hemicellulosic and cellulosic materials to valuable products such as fermentable sugars, biofuels, paper and other chemical products.Gram-positive, rod-shaped, catalase positive and endospore-forming bacteria were isolated by using selective and non selective conditions from samples of industrial liquors from active fermentation sugar cane bagasse piles in the North-West of Argentina. Among several others, these isolates are part of a complex consortium of organisms belonging to diverse eubacteria groups such as Bacteroides, Proteobacteria, Acidobacteria and other Firmicutes. In this study, 16S rRNA genes sequence analysis of selected isolates demonstrated that are close to moderately (< 96 - 91%) related to species of Paenibacillaceae family. Two wild-type isolates named AR92 and 109 are facultatively anaerobic, xylanolytic and closest to Cohnella, and Paenibacillus species, respectively, shown to be of industrial interest and were subject to different culture conditions to analyse their xylan degradation.