Agrowastes as Feedstock for the Production of Endo-β-Xylanase from Cohnella sp. Strain AR92

PISA, JOSÉ H.; MANFREDI, ADRIANA P.; PEROTTI, NORA I.; ROMERO, HÉCTOR G.; BRECCIA, JAVIER D.; MARTÍNEZ, MARÍA ALEJANDRA

JOURNAL OF MOLECULAR MICROBIOLOGY AND BIOTECHNOLOGY

KARGER

Año: 2017 p. 277 - 288

1464-1801

Members of Cohnella sp. isolated from a variety of environ- ments have been shown to be glycoside hydrolase producers. Nevertheless, most evaluations of members of this ge- nus are limited to their taxonomic description. The strain AR92, previously identified as belonging to the genus Cohnella, formed a well-supported cluster with C. thailand- ensis and C. formosensis (>80% bootstrap confidence). Its growth and xylanase production were approached by us- ing a mineral-based medium containing alkali-pretreated sugarcane bagasse as the main carbon source, which was assayed as a convenient source to produce biocatalysts po- tentially fitting its degradation. By means of a two-step statistical approach, the production of endoxylanase was mod- erately improved (20%). However, a far more significant improvement was observed (145%), by increasing the inoculum size and lowering the fermentation temperature to 25 ° C, which is below the optimal growth temperature of the strain AR92 (37 ° C). The xylanolytic preparation produced by Cohnella sp. AR92 contained mild temperature-active endoxylanase (identified as redundant GH10 family) for the main activity which resulted in xylobiose and xylo-oligo- saccharides as the main products from birchwood xylan.