INVESTIGADORES
MARTINEZ Maria Alejandra
artículos
Título:
Simple method for plasmid mediated transformation of different Bacillus species
Autor/es:
MARTINEZ M. ALEJANDRA; DEZAR A; BAIGORI MARIO D.; SINERIZ FAUSTINO
Revista:
BIOTECHNOLOGY TECHNIQUES
Editorial:
SPRINGER
Referencias:
Lugar: Netherlands; Año: 1999 p. 337 - 340
ISSN:
0951-208X
Resumen:
A simple and easy method for the introduction of plasmid DNA into different species of Bacillus was developed. The method involves the suspension in a transformation buffer of nutrient agar grown cells in their late exponential phase and the addition of unpurified plasmid DNA. Transformants were obtained at a frequency of about 103 to 105 stable transformants per g of plasmid DNA.
