INVESTIGADORES
SAPARRAT Mario Carlos Nazareno
congresos y reuniones científicas
Título:
Impact of a tandem treatment with a chemical oxidant and ligninolytic fungi augmentation on a petrochemical sludge
Autor/es:
DI CLEMENTE, NATALIA; PELUFFO, MARINA; MARIO CARLOS NAZARENO SAPARRAT; DEL PANNO, T.
Lugar:
Leipzig
Reunión:
Simposio; 17th International Symposium on Microbial Ecology; 2018
Institución organizadora:
ISME (International Society for Microbial Ecology)
Resumen:
Petrochemical sludges are a serious environmental problem generated by petroleum industry. The aim of this study was to analyse the combined effect of the chemical oxidation with ammonium persulfate (APS) followed by the augmentation with ligninolytic fungi (Coriolopsis rigida LPSC-232 and Grammothele subargentea LPSC-436) to remediate a petrochemical sludge. Microcosms of sludge and sand (1:3) were inoculated with each fungus separately using wheat straw as carrier, under axenic and non-sterile conditions. After 30 and 60 days of incubation, concentration of total hydrocarbons (IR) and alkane and PAH fractions (GC-FID) were determined. Dehydrogenase and laccase activities were evaluated and the PCR-DGGE technique and DNA sequencing were applied to analyse the microbial structure changes.Under axenic conditions with or without previous APS treatment, both fungi altered the availability of alkane and PAH fractions, probably due to the wheat straw degradation. Both hydrocarbon fractions were not detected in presence of the sludge microbiota. The oxidative treatment reduced 7% of the hydrocarbons content and 50% of the microbial density. Besides, laccase activity was detected in inoculated microcosms with both fungi in axenic and non-sterile conditions. Sequencing analysis of 16SrDNA and 18SrDNA from the sludge microcosms allowed the identification of the predominant taxa of the resilient populations, and those that settled after the bioaugmentation treatments. These results raise questions about the main contribution that ligninolytic fungi make in the transformation of petrochemical residues and their response to a previous oxidative chemical treatment, if applied, and to their interaction with indigenous sludge microbiota.
