CONICET | Buscador de Institutos y Recursos Humanos

Nowadays is considered that the success of biological strategies to remediate polluted sites with xenobiotics could be reached through microbial consortia. Several fungi such as ligninolytic ones can degrade and/or detoxify numerous recalcitrant compounds. According to the ability of mycelia from some fungi to act as highways for bacterial dispersion, we evaluated if ligninolytic fungi Coriolopsis rigida LPSC#232 and Grammothele subargentea LPSC#436, previously selected by their ability to grow and/or degrade pollutants, are able to isolate polycyclic aromatic hydrocarbons (PAH)-degrading bacteria from an oily sludge. We estimated fungal tolerance against different PAHs on agar medium and their hydrophobicity by contact angle. Bacterial isolation assay from oily sludge was evaluated in reversed minimal medium agar plate with wheat straw (MM), without PAH (control-I), MM with pyrene (PYR-II) and MM with PYR and dibenzothiophene (PYR+DBT-III).Colonized wheat straw by each fungus was put on sludge with a malt agar disc in the lid of upside-down placed plate. Uncolonized wheat straw was used as controls. All treatments were done in triplicates. After 45 days of incubation at room temperature and under dark conditions, bacterial colonies were detected only in association with LPSC#232/PYR+DBT. Bacteria was identified by morphology and 16S rRNA sequencing as Pseudomonas sp. Axenic assays showed its ability to degrade DBT on minimal medium. Cocultivation tests have been setted to corroborate the possible interaction between this bacterium and LPSC#232 to inquire if this consortium could be used in the context of bioaugmentation strategies to remediate polluted sites with oily sludges and other pollutants.

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