High throughput DNA sequencing of museum specimens sheds light on the long missing species of the Bokermannohyla claresignata group (Anura: Hylidae: Cophomantini)

LYRA, M.L.; LOURENÇO, ANA CAROLINA C.; PINHEIRO, P. D.; PEZZUTI, T.L.; BAETA, D.; BARLOW, AXEL; HOFREITER, M.; POMBAL, J. P., JR; HADDAD, C. F. B.; FAIVOVICH, J.

ZOOLOGICAL JOURNAL OF THE LINNEAN SOCIETY

WILEY-BLACKWELL PUBLISHING, INC

Lugar: Londres; Año: 2020 vol. 190 p. 1235 - 1255

0024-4082

The two species of the Bokermannohyla claresignata species group (Anura: Hylidae) have not been collected for the lastfour decades. It is the only species group of the hyline tribe Cophomantini that has not yet been analysed genetically.Its phylogenetic position is thus uncertain, and it has a combination of adult and larval character states that makethis group a crucial missing piece that hinders our understanding of Cophomantini phylogenetics and characterevolution. We obtained DNA sequences from a museum larval specimen of Bok. claresignata, using specializedextraction methods and high-throughput DNA sequencing, and combined the molecular phylogenetic results withavailable phenotypic information to provide new insights into the taxonomy and phylogenetic relationships of itsspecies group. Our phylogenetic results place Bok. claresignata as sister to the Boana pulchella group, supporting itsinclusion in Boana, together with Bokermannohyla clepsydra. In light of this new finding, we recognize a newly definedBoana claresignata group to accommodate these species, thus resolving both the polyphyly of Bokermannohyla andthe paraphyly of Boana. Considering the phylogenetic relationships of the Boana claresignata group, we also discussthe evolution of suctorial tadpoles and mature oocyte/egg pigmentation in Cophomantini.