Electrochemical magnetic microbeads-based biosensor for point-of-care serodiagnosis of infectious diseases

MARÍA EUGENIA CORTINA; LUCIANO MELLI; MARIANO ROBERTI; MIJAL MASS; GLORIA LONGINOTTI; SALVADOR TROPEA; PAULINA LLORET; REY SERANTES D.A.; FRANCISCO SALOMÓN; MATÍAS LLORET; CAILLAVA AJ; RESTUCCIA S; ALTCHEH J.; BUSCAGLIA C.A.; LAURA MALATTO; JUAN E. UGALDE; FRAIGI L.; CARLOS MOINA; GABRIEL YBARRA; ANDRÉS E. CIOCCHINI; DIEGO J. COMERCI

BIOSENSORS & BIOELECTRONICS

ELSEVIER ADVANCED TECHNOLOGY

Lugar: Amsterdam; Año: 2016 vol. 80 p. 24 - 33

0956-5663

Access toappropriatediagnostictoolsisanessentialcomponentintheevaluationandimprovementof global health.Additionally,timelydetectionofinfectiousagentsiscriticalinearlydiagnosisandtreat- ment ofinfectiousdiseases.Conventionalpathogendetectionmethodssuchasculturing,enzymelinked immunosorbent assay(ELISA)orpolymerasechainreaction(PCR)requirelongassaytimes,andcomplex and expensiveinstrumentsmakingthemnotadaptabletopoint-of-care(PoC)needsatresource-con- strained placesandprimarycaresettings.Therefore,thereisanunmetneedtodevelopportable,simple, rapid, andaccuratemethodsforPoCdetectionofinfections.Here,wepresentthedevelopmentand validationofaportable,robustandinexpensiveelectrochemicalmagneticmicrobeads-basedbiosensor (EMBIA) platformforPoCserodiagnosisofinfectiousdiseasescausedbydifferenttypesofmicroorgan- isms (parasiticprotozoa,bacteriaandviruses).WedemonstratethepotentialuseoftheEMBIAplatform for in situ diagnosis ofhuman(Chagasdiseaseandhumanbrucellosis)andanimal(bovinebrucellosisand foot-and-mouth disease)infectionsclearlydifferentiatinginfectedfromnon-infectedindividualsoran- imals. ForChagasdisease,amoreextensivevalidationofthetestwasperformedshowingthattheEMBIA platform displayedanexcellentdiagnosticperformancealmostindistinguishable,intermsofspecificity and sensitivity,froma fluorescent immunomagneticassayandtheconventionalELISAusingthesame combination ofantigens.Thisplatformtechnologycouldpotentiallybeapplicabletodiagnoseother infectious andnon-infectiousdiseasesaswellasdetectionand/orquantification ofbiomarkersatthe POC andprimarycaresettings.