INVESTIGADORES
COMERCI Diego Jose
artículos
Título:
A bacterial engineered glycoprotein as a novel antigen for diagnosis of bovine brucellosis
Autor/es:
CIOCCHINI AE; REY SERANTES DA; MELLI LJ; GUIDOLIN L. S.; IWASHKIW JA; ELENA S.; FRANCO C.; NICOLA A.M.; FELDMAN MF; COMERCI DJ; UGALDE JE
Revista:
VETERINARY MICROBIOLOGY
Editorial:
ELSEVIER SCIENCE BV
Referencias:
Lugar: Amsterdam; Año: 2014 vol. 172 p. 455 - 465
ISSN:
0378-1135
Resumen:
Brucellosis is a highly contagious zoonosis that affects livestock and human beings.
Laboratory diagnosis of bovine brucellosis mainly relies on serological diagnosis using
serum and/or milk samples. Although there are several serological tests with different
diagnostic performance and capacity to differentiate vaccinated from infected animals,
there is still no standardized reference antigen for the disease. Here we validate the first
recombinant glycoprotein antigen, an N-formylperosamine O-polysaccharide-protein
conjugate (OAg-AcrA), for diagnosis of bovine brucellosis. This antigen can be produced in
homogeneous batches without the need of culturing pathogenic brucellae; all characteristics
that make it appropriate for standardization. An indirect immunoassay based on the
detection of anti O-polysaccharide IgG antibodies in bovine samples was developed coupling
OAg-AcrA to magnetic beads or ELISA plates. As a proof of concept and to validate the antigen,
we analyzed serum, whole blood and milk samples obtained from non-infected,
experimentally infected and vaccinated animals included in a vaccination/infection trial
performed in our laboratory as well as more than 1000 serum and milk samples obtained
from naturally infected and S19-vaccinated animals from Argentina. Our results demonstrate
that OAg-AcrA-based assays are highly accurate for diagnosis of bovine brucellosis, even in
vaccinated herds, using different types of samples and in different platforms. We propose this
novel recombinant glycoprotein as an antigen suitable for the development of new standard
immunological tests for screening and confirmatory diagnosis of bovine brucellosis in regions
or countries with brucellosis-control programs