INVESTIGADORES
MARTIN Valentina
congresos y reuniones científicas
Título:
Acute Toxoplasma gondii infection modulates systemic immune response against allergen in a murine model of allergic airway inflammation
Autor/es:
FENOY, IGNACIO; CHIURAZZI, ROMINA; SÁNCHEZ, VANESA; CASTERA, FACUNDO; MARTÍN, VALENTINA; GOLDMAN, ALEJANDRA
Reunión:
Congreso; First French Argentine Immunology Congress; 2010
Institución organizadora:
SAI
Resumen:
We
previously showed that acute infection with T.gondii
blocks the development of allergic
lung inflammation. OVA-sensitization during acute phase results in lower
levels of OVA-specific IgE, IgG1 and
Th2 cytokines from thoracic lymph nodes (LN). The increase in OVA-specific
IgG2a and IFN-γ levels from OVA stimulated LN cells suggests that parasite
infection induces a shift towards a Th1 response. Nevertheless,
regulatory mechanisms could also be implicated since increases in TGF-β levels
are detected. IL-10 behaves as a typical Th2 cytokine. The aim of the present
study was to evaluate the systemic levels of Th1, Th2 and regulatory cytokines.
Adult BALB/c mice were ip
sensitized and aerosol challenged with OVA(O). One week before sensitization,
the experimental group (OT) was orally infected with cysts from Beverly strain.
Two days post challenge, splenocytes were in
vitro stimulated with OVA or total lysate T.gondii antigen (TLA) and supernatant cytokine levels were
evaluated. Sensitization during acute T.gondii
infection induced a systemic decrease in OVA specific IL-4 (pg/ml: N:ND; T:ND;
O:1341±198a; OT: 469±252) and IL-10 (pg/ml: N:ND; T: 28±28; O: 642±7 c; OT: 371±193c).
An increase in allergen specific IFN-γ levels in OT compared to the O group was detected (pg/ml: N:ND; T:ND;
O: 106±27a; OT: 322±4a).
TGF-β levels showed no
significant diferences. Nevertheless, a trend toward increased TGF-β production was observed in the OT
group (OD: N: 0,02±0,09; T:0,06±0,02; O: 0,07±0,03; OT: 0,13±0,05). IFN-g and IL-10 levels in the infected groups
stimulated with TLA are those expected for the
immune response against T.gondii (IFNg pg/ml: N: 57±57; T: 850±67b; O: 13±4;
OT: 694±235b;
IL-10: N: 133±93; T: 720±265; O: 29±15; OT: 748±295). (Mean±SEM. a
P<0,05 vs all groups; b P<0,05 vs N y O; c
P<0,05 vs N y T. ND: not detected). These results show that systemic cytokine
profile correlates with the one observed in lung draining LN