IMMUNE-MODULATORY EFFECTS OF THE SERINE PROTEASE INHIBITOR-1 OF Toxoplasma gondii ON INNATE IMMUNE RESPONSE

VANESA SÁNCHEZ, MATÍAS PERRONE SIBILIAC, MARIANO PICCHIO, NADIA ARCON, ARIADNA SOTTO, MARÍA DE LOS ANGELES ALDIRICO, IGNACIO FENOY, ALEJANDRA GOLDMAN, VALENTINA MARTIN

Congreso; Reunión Conjunta de Sociedades de Biociencias; 2017

Toxoplasma gondii infects approximately one-third of the worldpopulation, making it one of the most successful parasitic organisms.T. gondii serine protease inhibitor-1 (TgPI-1) is a potent inhibitor ofneutrophil elastase, trypsin and chymotrypsin but its role during naturalinfection is not already known. It has been reported that other serine protease inhibitors are able to modulate the immune system.Previously we showed that TgPI-1 was able to down-modulate botha Th2 and Th1 type immune responses already established. Thoseresults led us to explore the ability of TgPI-1 to modulate the innateimmune response. In this work we used the J774-A1 murine macrophagecell line to study this possible modulatory role. Cells werestimulated with lipopolysaccharide (LPS) or exposed to residual oilfly ash (ROFA), in the presence of a recombinant form of TgPI-1(rTgPI-1). We observed that rTgPI-1 was able to decrease nitric oxideproduction induced by LPS stimulation when compared to LPSalone (p