Preliminar data on growth and enzyme abilities of soil fungus Humicolopsis cephalosporioides under different incubation temperatures.

ELÍADES, L A, ; CABELLO MN, ; PANCOTTO VA; ; MORETTO, A, ; RAGO MM, ; SAPARRAT MCN

REVISTA IBEROAMERICANA DE MICOLOGIA

ASOCIACION ESPANOLA MICOLOGIA-AEM

Lugar: Londres; Año: 2015 vol. 32 p. 40 - 45

1130-1406

Abstract: Background: Nothofagus pumilio (Poepp & Endl.) Krasser "lenga" is the most important timber wood species in the southernmost Patagonia (Argentina). Humicolopsis cephalosporioides Cabral & Marchand is a soil fungus associated to Nothofagus pumilio forests, which has an outstanding cellulolytic activity. However there is no information about the ability of this fungus to use other organic substrates than cellulose, and to produce different enzyme systems as well as its response to temperature. Objectives: The aim of this study was to get a better insight into the role of H. cephalosporioides in degradation processes in N. pumilio forests by evaluating the ability in-vitro of four isolates of this fungus to grow and produce different lytic enzyme systems and its response to incubation temperature. Methods: The ability of fungi to grow and produce enzyme systems was estimated inoculating them on agar media with specific substrates, being cultures incubated at three temperatures. Results: A differential behavior of each strain in levels of growth and enzyme activity was found according to the medium type and/or incubation temperature. Conclussions: A intraspecific variability was found in H. cephalosporioides. Likewise a possible link between saprotrophic role of this fungus in Nothofagus pumilio forests and degradation of organic matter under stressfull conditions such as ones from frost environments was also discussed.