Development of microsatellite markers for the American species Vachellia aroma (Fabaceae, Caesalpinodeae)

POMETTI, CAROLINA; BESSEGA C; EWENS M; VILARDI J. C.; SAIDMAN B. O.

BOLETíN DE LA SOCIEDAD ARGENTINA DE BOTáNICA

SOCIEDAD ARGENTINA DE BOTÁNICA

Lugar: Córdoba; Año: 2024

0373-580X

Background and aims: There are currently no microsatellite markers available for anyAmerican species of Vachellia, and particularly, for V. aroma. Then the aims of thisstudy were to develop SSR markers specific for V. aroma, for the first time, and testits amplification in a close related species.M&M: For the development of the SSR in V. aroma, total genomic DNA was extractedand it was sequenced in a one-fourth run on a Roche 454 GS FLX+ platform. Thestudy area included two Argentinean populations: San José and Robles, Santiagodel Estero province.Results: We detected 422 sequences containing SSR loci. A set of 39 primer pairspresented amplified products in V. aroma and V. caven, but 12 revealed clear,replicable and polymorphic loci in V. aroma.Conclusions: The results of this work indicate that a new set of SSR markerswas developed for V. aroma and their transferability to V. caven was assessed.The analysis of variability, showed that these 12 polymorphic markers are highlyinformative, and a powerful tool to investigate population genetics parameters in V.aroma and related species.