Study of placental cells behavior under PTHrP effect and other factors of the cellular environment

NOVOA DÍAZ MARÍA BELEN; CARRIERE PEDRO; BIRKENSTOK CINTIA; MACCHI ROSARIO; CALVO NATALIA; SICA GABRIELA; CANELLADA ANDREA; VINDEROLA GABRIEL; GENTILI CLAUDIA

Congreso; Reunión conjunta SAIC-SAB-AAFE-AACYTAL; 2023

SAIC-SAB-AAFE-AACYTAL

Through paracrine signals, trophoblast cells invade the endometrium in early placentation. Thus, an unbalanced influence of environmental, maternal, and fetal factors on these cells can lead to gestational diseases. Parathyroid hormone-related peptide (PTHrP) expressed in the placenta may be one of these factors. It promotes epithelial-mesenchymal transition (EMT) and invasion, phenomena associated with physiological and pathological events. Other factors that could influence on placental structure and function are those derived from gut microbiota; in fact Bifidobacterium is now an attractive genus for its study in the gestational stage. This work aims to investigate if PTHrP influences trophoblastic cells phenotype and if so, whether human milk-derived Bifidobacterium animalis subsp lactis INL1 (B. lactis INL1) is able to participate in its effect.We used HTR-8 and Bewo cells from normal placenta and choriocarcinoma, respectively, whose express PTHrP receptor, as revealed an in silico analysis. PTHrP at physiological concentration (10-12M) induces EMT-associated phenotype in HTR-8 cells (p