INVESTIGADORES
GENTILI Claudia Rosana
artículos
Título:
Age-related decline in mitogen-activated protein kinase phosphorylation in PTH- stimulated rat enterocytes
Autor/es:
GENTILI C; RUSSO DE BOLAND A
Revista:
EXPERIMENTAL GERONTOLOGY
Editorial:
PERGAMON-ELSEVIER SCIENCE LTD
Referencias:
Año: 2000 vol. 35 p. 1003 - 1015
ISSN:
0531-5565
Resumen:
In the present study we analyzed whether parathyroid hormone (rPTH[1-34]; PTH) stimulates the
tyrosine phosphorylation of the growth-related protein mitogen-activated protein (MAP) kinases
(p42/44-MAPK), also known as extracellular signal-regulated kinases (ERK1/2), in duodenal
enterocytes isolated from young (3 months) and aged (24 months) rats. Western blot analysis
revealed that PTH rapidly stimulates MAPK phosphorylation. The hormone effects on MAPK
were evident within 30 s, peaking at 1 min (4-fold). PTH response was dose-dependent (10-7
10-11 M) with maximal stimulation achieved at 10-910-8 M. PTH-induced MAPK phosphorylation
was effectively suppressed by the tyrosine-kinase inhibitors, genistein (100 mM) and herbimycin
(2 mM). Moreover, the tyrosine phosphorylation and activation of MAPK was dependent on Src
kinase, since PP1 (10 and 20 mM), a specific Src family tyrosine-kinase inhibitor, blocked PTHinduced
MAPK activation. With aging, the response to PTH was significantly reduced. However,
The amount of basal protein expression determined by Western blot analysis for MAPK was not
different in the enterocytes from young and aged rats.
In conclusion, the results obtained in this work expand our knowledge on the mechanism of action
of PTH in duodenal cells, revealing that protein tyrosine phosphorylation is linked to the PTH
regulation of enterocyte MAPK activation, and that this mechanism is impaired with aging.
Understanding the molecular mechanisms for the age-related differences in PTH signaling will
require more information about the subtle mechanisms that modulate the PTH receptor-MAPK
signaling pathway.
MAPK activation. With aging, the response to PTH was significantly reduced. However,
The amount of basal protein expression determined by Western blot analysis for MAPK was not
different in the enterocytes from young and aged rats.
In conclusion, the results obtained in this work expand our knowledge on the mechanism of action
of PTH in duodenal cells, revealing that protein tyrosine phosphorylation is linked to the PTH
regulation of enterocyte MAPK activation, and that this mechanism is impaired with aging.
Understanding the molecular mechanisms for the age-related differences in PTH signaling will
require more information about the subtle mechanisms that modulate the PTH receptor-MAPK
signaling pathway.
kinase, since PP1 (10 and 20 mM), a specific Src family tyrosine-kinase inhibitor, blocked PTHinduced
MAPK activation. With aging, the response to PTH was significantly reduced. However,
The amount of basal protein expression determined by Western blot analysis for MAPK was not
different in the enterocytes from young and aged rats.
In conclusion, the results obtained in this work expand our knowledge on the mechanism of action
of PTH in duodenal cells, revealing that protein tyrosine phosphorylation is linked to the PTH
regulation of enterocyte MAPK activation, and that this mechanism is impaired with aging.
Understanding the molecular mechanisms for the age-related differences in PTH signaling will
require more information about the subtle mechanisms that modulate the PTH receptor-MAPK
signaling pathway.
MAPK activation. With aging, the response to PTH was significantly reduced. However,
The amount of basal protein expression determined by Western blot analysis for MAPK was not
different in the enterocytes from young and aged rats.
In conclusion, the results obtained in this work expand our knowledge on the mechanism of action
of PTH in duodenal cells, revealing that protein tyrosine phosphorylation is linked to the PTH
regulation of enterocyte MAPK activation, and that this mechanism is impaired with aging.
Understanding the molecular mechanisms for the age-related differences in PTH signaling will
require more information about the subtle mechanisms that modulate the PTH receptor-MAPK
signaling pathway.
(2 mM). Moreover, the tyrosine phosphorylation and activation of MAPK was dependent on Src
kinase, since PP1 (10 and 20 mM), a specific Src family tyrosine-kinase inhibitor, blocked PTHinduced
MAPK activation. With aging, the response to PTH was significantly reduced. However,
The amount of basal protein expression determined by Western blot analysis for MAPK was not
different in the enterocytes from young and aged rats.
In conclusion, the results obtained in this work expand our knowledge on the mechanism of action
of PTH in duodenal cells, revealing that protein tyrosine phosphorylation is linked to the PTH
regulation of enterocyte MAPK activation, and that this mechanism is impaired with aging.
Understanding the molecular mechanisms for the age-related differences in PTH signaling will
require more information about the subtle mechanisms that modulate the PTH receptor-MAPK
signaling pathway.
MAPK activation. With aging, the response to PTH was significantly reduced. However,
The amount of basal protein expression determined by Western blot analysis for MAPK was not
different in the enterocytes from young and aged rats.
In conclusion, the results obtained in this work expand our knowledge on the mechanism of action
of PTH in duodenal cells, revealing that protein tyrosine phosphorylation is linked to the PTH
regulation of enterocyte MAPK activation, and that this mechanism is impaired with aging.
Understanding the molecular mechanisms for the age-related differences in PTH signaling will
require more information about the subtle mechanisms that modulate the PTH receptor-MAPK
signaling pathway.
kinase, since PP1 (10 and 20 mM), a specific Src family tyrosine-kinase inhibitor, blocked PTHinduced
MAPK activation. With aging, the response to PTH was significantly reduced. However,
The amount of basal protein expression determined by Western blot analysis for MAPK was not
different in the enterocytes from young and aged rats.
In conclusion, the results obtained in this work expand our knowledge on the mechanism of action
of PTH in duodenal cells, revealing that protein tyrosine phosphorylation is linked to the PTH
regulation of enterocyte MAPK activation, and that this mechanism is impaired with aging.
Understanding the molecular mechanisms for the age-related differences in PTH signaling will
require more information about the subtle mechanisms that modulate the PTH receptor-MAPK
signaling pathway.
MAPK activation. With aging, the response to PTH was significantly reduced. However,
The amount of basal protein expression determined by Western blot analysis for MAPK was not
different in the enterocytes from young and aged rats.
In conclusion, the results obtained in this work expand our knowledge on the mechanism of action
of PTH in duodenal cells, revealing that protein tyrosine phosphorylation is linked to the PTH
regulation of enterocyte MAPK activation, and that this mechanism is impaired with aging.
Understanding the molecular mechanisms for the age-related differences in PTH signaling will
require more information about the subtle mechanisms that modulate the PTH receptor-MAPK
signaling pathway.
was effectively suppressed by the tyrosine-kinase inhibitors, genistein (100 mM) and herbimycin
(2 mM). Moreover, the tyrosine phosphorylation and activation of MAPK was dependent on Src
kinase, since PP1 (10 and 20 mM), a specific Src family tyrosine-kinase inhibitor, blocked PTHinduced
MAPK activation. With aging, the response to PTH was significantly reduced. However,
The amount of basal protein expression determined by Western blot analysis for MAPK was not
different in the enterocytes from young and aged rats.
In conclusion, the results obtained in this work expand our knowledge on the mechanism of action
of PTH in duodenal cells, revealing that protein tyrosine phosphorylation is linked to the PTH
regulation of enterocyte MAPK activation, and that this mechanism is impaired with aging.
Understanding the molecular mechanisms for the age-related differences in PTH signaling will
require more information about the subtle mechanisms that modulate the PTH receptor-MAPK
signaling pathway.
MAPK activation. With aging, the response to PTH was significantly reduced. However,
The amount of basal protein expression determined by Western blot analysis for MAPK was not
different in the enterocytes from young and aged rats.
In conclusion, the results obtained in this work expand our knowledge on the mechanism of action
of PTH in duodenal cells, revealing that protein tyrosine phosphorylation is linked to the PTH
regulation of enterocyte MAPK activation, and that this mechanism is impaired with aging.
Understanding the molecular mechanisms for the age-related differences in PTH signaling will
require more information about the subtle mechanisms that modulate the PTH receptor-MAPK
signaling pathway.
kinase, since PP1 (10 and 20 mM), a specific Src family tyrosine-kinase inhibitor, blocked PTHinduced
MAPK activation. With aging, the response to PTH was significantly reduced. However,
The amount of basal protein expression determined by Western blot analysis for MAPK was not
different in the enterocytes from young and aged rats.
In conclusion, the results obtained in this work expand our knowledge on the mechanism of action
of PTH in duodenal cells, revealing that protein tyrosine phosphorylation is linked to the PTH
regulation of enterocyte MAPK activation, and that this mechanism is impaired with aging.
Understanding the molecular mechanisms for the age-related differences in PTH signaling will
require more information about the subtle mechanisms that modulate the PTH receptor-MAPK
signaling pathway.
MAPK activation. With aging, the response to PTH was significantly reduced. However,
The amount of basal protein expression determined by Western blot analysis for MAPK was not
different in the enterocytes from young and aged rats.
In conclusion, the results obtained in this work expand our knowledge on the mechanism of action
of PTH in duodenal cells, revealing that protein tyrosine phosphorylation is linked to the PTH
regulation of enterocyte MAPK activation, and that this mechanism is impaired with aging.
Understanding the molecular mechanisms for the age-related differences in PTH signaling will
require more information about the subtle mechanisms that modulate the PTH receptor-MAPK
signaling pathway.
(2 mM). Moreover, the tyrosine phosphorylation and activation of MAPK was dependent on Src
kinase, since PP1 (10 and 20 mM), a specific Src family tyrosine-kinase inhibitor, blocked PTHinduced
MAPK activation. With aging, the response to PTH was significantly reduced. However,
The amount of basal protein expression determined by Western blot analysis for MAPK was not
different in the enterocytes from young and aged rats.
In conclusion, the results obtained in this work expand our knowledge on the mechanism of action
of PTH in duodenal cells, revealing that protein tyrosine phosphorylation is linked to the PTH
regulation of enterocyte MAPK activation, and that this mechanism is impaired with aging.
Understanding the molecular mechanisms for the age-related differences in PTH signaling will
require more information about the subtle mechanisms that modulate the PTH receptor-MAPK
signaling pathway.
MAPK activation. With aging, the response to PTH was significantly reduced. However,
The amount of basal protein expression determined by Western blot analysis for MAPK was not
different in the enterocytes from young and aged rats.
In conclusion, the results obtained in this work expand our knowledge on the mechanism of action
of PTH in duodenal cells, revealing that protein tyrosine phosphorylation is linked to the PTH
regulation of enterocyte MAPK activation, and that this mechanism is impaired with aging.
Understanding the molecular mechanisms for the age-related differences in PTH signaling will
require more information about the subtle mechanisms that modulate the PTH receptor-MAPK
signaling pathway.
kinase, since PP1 (10 and 20 mM), a specific Src family tyrosine-kinase inhibitor, blocked PTHinduced
MAPK activation. With aging, the response to PTH was significantly reduced. However,
The amount of basal protein expression determined by Western blot analysis for MAPK was not
different in the enterocytes from young and aged rats.
In conclusion, the results obtained in this work expand our knowledge on the mechanism of action
of PTH in duodenal cells, revealing that protein tyrosine phosphorylation is linked to the PTH
regulation of enterocyte MAPK activation, and that this mechanism is impaired with aging.
Understanding the molecular mechanisms for the age-related differences in PTH signaling will
require more information about the subtle mechanisms that modulate the PTH receptor-MAPK
signaling pathway.
MAPK activation. With aging, the response to PTH was significantly reduced. However,
The amount of basal protein expression determined by Western blot analysis for MAPK was not
different in the enterocytes from young and aged rats.
In conclusion, the results obtained in this work expand our knowledge on the mechanism of action
of PTH in duodenal cells, revealing that protein tyrosine phosphorylation is linked to the PTH
regulation of enterocyte MAPK activation, and that this mechanism is impaired with aging.
Understanding the molecular mechanisms for the age-related differences in PTH signaling will
require more information about the subtle mechanisms that modulate the PTH receptor-MAPK
signaling pathway.
10-11 M) with maximal stimulation achieved at 10-910-8 M. PTH-induced MAPK phosphorylation
was effectively suppressed by the tyrosine-kinase inhibitors, genistein (100 mM) and herbimycin
(2 mM). Moreover, the tyrosine phosphorylation and activation of MAPK was dependent on Src
kinase, since PP1 (10 and 20 mM), a specific Src family tyrosine-kinase inhibitor, blocked PTHinduced
MAPK activation. With aging, the response to PTH was significantly reduced. However,
The amount of basal protein expression determined by Western blot analysis for MAPK was not
different in the enterocytes from young and aged rats.
In conclusion, the results obtained in this work expand our knowledge on the mechanism of action
of PTH in duodenal cells, revealing that protein tyrosine phosphorylation is linked to the PTH
regulation of enterocyte MAPK activation, and that this mechanism is impaired with aging.
Understanding the molecular mechanisms for the age-related differences in PTH signaling will
require more information about the subtle mechanisms that modulate the PTH receptor-MAPK
signaling pathway.
MAPK activation. With aging, the response to PTH was significantly reduced. However,
The amount of basal protein expression determined by Western blot analysis for MAPK was not
different in the enterocytes from young and aged rats.
In conclusion, the results obtained in this work expand our knowledge on the mechanism of action
of PTH in duodenal cells, revealing that protein tyrosine phosphorylation is linked to the PTH
regulation of enterocyte MAPK activation, and that this mechanism is impaired with aging.
Understanding the molecular mechanisms for the age-related differences in PTH signaling will
require more information about the subtle mechanisms that modulate the PTH receptor-MAPK
signaling pathway.
kinase, since PP1 (10 and 20 mM), a specific Src family tyrosine-kinase inhibitor, blocked PTHinduced
MAPK activation. With aging, the response to PTH was significantly reduced. However,
The amount of basal protein expression determined by Western blot analysis for MAPK was not
different in the enterocytes from young and aged rats.
In conclusion, the results obtained in this work expand our knowledge on the mechanism of action
of PTH in duodenal cells, revealing that protein tyrosine phosphorylation is linked to the PTH
regulation of enterocyte MAPK activation, and that this mechanism is impaired with aging.
Understanding the molecular mechanisms for the age-related differences in PTH signaling will
require more information about the subtle mechanisms that modulate the PTH receptor-MAPK
signaling pathway.
MAPK activation. With aging, the response to PTH was significantly reduced. However,
The amount of basal protein expression determined by Western blot analysis for MAPK was not
different in the enterocytes from young and aged rats.
In conclusion, the results obtained in this work expand our knowledge on the mechanism of action
of PTH in duodenal cells, revealing that protein tyrosine phosphorylation is linked to the PTH
regulation of enterocyte MAPK activation, and that this mechanism is impaired with aging.
Understanding the molecular mechanisms for the age-related differences in PTH signaling will
require more information about the subtle mechanisms that modulate the PTH receptor-MAPK
signaling pathway.
(2 mM). Moreover, the tyrosine phosphorylation and activation of MAPK was dependent on Src
kinase, since PP1 (10 and 20 mM), a specific Src family tyrosine-kinase inhibitor, blocked PTHinduced
MAPK activation. With aging, the response to PTH was significantly reduced. However,
The amount of basal protein expression determined by Western blot analysis for MAPK was not
different in the enterocytes from young and aged rats.
In conclusion, the results obtained in this work expand our knowledge on the mechanism of action
of PTH in duodenal cells, revealing that protein tyrosine phosphorylation is linked to the PTH
regulation of enterocyte MAPK activation, and that this mechanism is impaired with aging.
Understanding the molecular mechanisms for the age-related differences in PTH signaling will
require more information about the subtle mechanisms that modulate the PTH receptor-MAPK
signaling pathway.
MAPK activation. With aging, the response to PTH was significantly reduced. However,
The amount of basal protein expression determined by Western blot analysis for MAPK was not
different in the enterocytes from young and aged rats.
In conclusion, the results obtained in this work expand our knowledge on the mechanism of action
of PTH in duodenal cells, revealing that protein tyrosine phosphorylation is linked to the PTH
regulation of enterocyte MAPK activation, and that this mechanism is impaired with aging.
Understanding the molecular mechanisms for the age-related differences in PTH signaling will
require more information about the subtle mechanisms that modulate the PTH receptor-MAPK
signaling pathway.
kinase, since PP1 (10 and 20 mM), a specific Src family tyrosine-kinase inhibitor, blocked PTHinduced
MAPK activation. With aging, the response to PTH was significantly reduced. However,
The amount of basal protein expression determined by Western blot analysis for MAPK was not
different in the enterocytes from young and aged rats.
In conclusion, the results obtained in this work expand our knowledge on the mechanism of action
of PTH in duodenal cells, revealing that protein tyrosine phosphorylation is linked to the PTH
regulation of enterocyte MAPK activation, and that this mechanism is impaired with aging.
Understanding the molecular mechanisms for the age-related differences in PTH signaling will
require more information about the subtle mechanisms that modulate the PTH receptor-MAPK
signaling pathway.
MAPK activation. With aging, the response to PTH was significantly reduced. However,
The amount of basal protein expression determined by Western blot analysis for MAPK was not
different in the enterocytes from young and aged rats.
In conclusion, the results obtained in this work expand our knowledge on the mechanism of action
of PTH in duodenal cells, revealing that protein tyrosine phosphorylation is linked to the PTH
regulation of enterocyte MAPK activation, and that this mechanism is impaired with aging.
Understanding the molecular mechanisms for the age-related differences in PTH signaling will
require more information about the subtle mechanisms that modulate the PTH receptor-MAPK
signaling pathway.
was effectively suppressed by the tyrosine-kinase inhibitors, genistein (100 mM) and herbimycin
(2 mM). Moreover, the tyrosine phosphorylation and activation of MAPK was dependent on Src
kinase, since PP1 (10 and 20 mM), a specific Src family tyrosine-kinase inhibitor, blocked PTHinduced
MAPK activation. With aging, the response to PTH was significantly reduced. However,
The amount of basal protein expression determined by Western blot analysis for MAPK was not
different in the enterocytes from young and aged rats.
In conclusion, the results obtained in this work expand our knowledge on the mechanism of action
of PTH in duodenal cells, revealing that protein tyrosine phosphorylation is linked to the PTH
regulation of enterocyte MAPK activation, and that this mechanism is impaired with aging.
Understanding the molecular mechanisms for the age-related differences in PTH signaling will
require more information about the subtle mechanisms that modulate the PTH receptor-MAPK
signaling pathway.
MAPK activation. With aging, the response to PTH was significantly reduced. However,
The amount of basal protein expression determined by Western blot analysis for MAPK was not
different in the enterocytes from young and aged rats.
In conclusion, the results obtained in this work expand our knowledge on the mechanism of action
of PTH in duodenal cells, revealing that protein tyrosine phosphorylation is linked to the PTH
regulation of enterocyte MAPK activation, and that this mechanism is impaired with aging.
Understanding the molecular mechanisms for the age-related differences in PTH signaling will
require more information about the subtle mechanisms that modulate the PTH receptor-MAPK
signaling pathway.
kinase, since PP1 (10 and 20 mM), a specific Src family tyrosine-kinase inhibitor, blocked PTHinduced
MAPK activation. With aging, the response to PTH was significantly reduced. However,
The amount of basal protein expression determined by Western blot analysis for MAPK was not
different in the enterocytes from young and aged rats.
In conclusion, the results obtained in this work expand our knowledge on the mechanism of action
of PTH in duodenal cells, revealing that protein tyrosine phosphorylation is linked to the PTH
regulation of enterocyte MAPK activation, and that this mechanism is impaired with aging.
Understanding the molecular mechanisms for the age-related differences in PTH signaling will
require more information about the subtle mechanisms that modulate the PTH receptor-MAPK
signaling pathway.
MAPK activation. With aging, the response to PTH was significantly reduced. However,
The amount of basal protein expression determined by Western blot analysis for MAPK was not
different in the enterocytes from young and aged rats.
In conclusion, the results obtained in this work expand our knowledge on the mechanism of action
of PTH in duodenal cells, revealing that protein tyrosine phosphorylation is linked to the PTH
regulation of enterocyte MAPK activation, and that this mechanism is impaired with aging.
Understanding the molecular mechanisms for the age-related differences in PTH signaling will
require more information about the subtle mechanisms that modulate the PTH receptor-MAPK
signaling pathway.
(2 mM). Moreover, the tyrosine phosphorylation and activation of MAPK was dependent on Src
kinase, since PP1 (10 and 20 mM), a specific Src family tyrosine-kinase inhibitor, blocked PTHinduced
MAPK activation. With aging, the response to PTH was significantly reduced. However,
The amount of basal protein expression determined by Western blot analysis for MAPK was not
different in the enterocytes from young and aged rats.
In conclusion, the results obtained in this work expand our knowledge on the mechanism of action
of PTH in duodenal cells, revealing that protein tyrosine phosphorylation is linked to the PTH
regulation of enterocyte MAPK activation, and that this mechanism is impaired with aging.
Understanding the molecular mechanisms for the age-related differences in PTH signaling will
require more information about the subtle mechanisms that modulate the PTH receptor-MAPK
signaling pathway.
MAPK activation. With aging, the response to PTH was significantly reduced. However,
The amount of basal protein expression determined by Western blot analysis for MAPK was not
different in the enterocytes from young and aged rats.
In conclusion, the results obtained in this work expand our knowledge on the mechanism of action
of PTH in duodenal cells, revealing that protein tyrosine phosphorylation is linked to the PTH
regulation of enterocyte MAPK activation, and that this mechanism is impaired with aging.
Understanding the molecular mechanisms for the age-related differences in PTH signaling will
require more information about the subtle mechanisms that modulate the PTH receptor-MAPK
signaling pathway.
kinase, since PP1 (10 and 20 mM), a specific Src family tyrosine-kinase inhibitor, blocked PTHinduced
MAPK activation. With aging, the response to PTH was significantly reduced. However,
The amount of basal protein expression determined by Western blot analysis for MAPK was not
different in the enterocytes from young and aged rats.
In conclusion, the results obtained in this work expand our knowledge on the mechanism of action
of PTH in duodenal cells, revealing that protein tyrosine phosphorylation is linked to the PTH
regulation of enterocyte MAPK activation, and that this mechanism is impaired with aging.
Understanding the molecular mechanisms for the age-related differences in PTH signaling will
require more information about the subtle mechanisms that modulate the PTH receptor-MAPK
signaling pathway.
MAPK activation. With aging, the response to PTH was significantly reduced. However,
The amount of basal protein expression determined by Western blot analysis for MAPK was not
different in the enterocytes from young and aged rats.
In conclusion, the results obtained in this work expand our knowledge on the mechanism of action
of PTH in duodenal cells, revealing that protein tyrosine phosphorylation is linked to the PTH
regulation of enterocyte MAPK activation, and that this mechanism is impaired with aging.
Understanding the molecular mechanisms for the age-related differences in PTH signaling will
require more information about the subtle mechanisms that modulate the PTH receptor-MAPK
signaling pathway.
10-11 M) with maximal stimulation achieved at 10-910-8 M. PTH-induced MAPK phosphorylation
was effectively suppressed by the tyrosine-kinase inhibitors, genistein (100 mM) and herbimycin
(2 mM). Moreover, the tyrosine phosphorylation and activation of MAPK was dependent on Src
kinase, since PP1 (10 and 20 mM), a specific Src family tyrosine-kinase inhibitor, blocked PTHinduced
MAPK activation. With aging, the response to PTH was significantly reduced. However,
The amount of basal protein expression determined by Western blot analysis for MAPK was not
different in the enterocytes from young and aged rats.
In conclusion, the results obtained in this work expand our knowledge on the mechanism of action
of PTH in duodenal cells, revealing that protein tyrosine phosphorylation is linked to the PTH
regulation of enterocyte MAPK activation, and that this mechanism is impaired with aging.
Understanding the molecular mechanisms for the age-related differences in PTH signaling will
require more information about the subtle mechanisms that modulate the PTH receptor-MAPK
signaling pathway.
MAPK activation. With aging, the response to PTH was significantly reduced. However,
The amount of basal protein expression determined by Western blot analysis for MAPK was not
different in the enterocytes from young and aged rats.
In conclusion, the results obtained in this work expand our knowledge on the mechanism of action
of PTH in duodenal cells, revealing that protein tyrosine phosphorylation is linked to the PTH
regulation of enterocyte MAPK activation, and that this mechanism is impaired with aging.
Understanding the molecular mechanisms for the age-related differences in PTH signaling will
require more information about the subtle mechanisms that modulate the PTH receptor-MAPK
signaling pathway.
kinase, since PP1 (10 and 20 mM), a specific Src family tyrosine-kinase inhibitor, blocked PTHinduced
MAPK activation. With aging, the response to PTH was significantly reduced. However,
The amount of basal protein expression determined by Western blot analysis for MAPK was not
different in the enterocytes from young and aged rats.
In conclusion, the results obtained in this work expand our knowledge on the mechanism of action
of PTH in duodenal cells, revealing that protein tyrosine phosphorylation is linked to the PTH
regulation of enterocyte MAPK activation, and that this mechanism is impaired with aging.
Understanding the molecular mechanisms for the age-related differences in PTH signaling will
require more information about the subtle mechanisms that modulate the PTH receptor-MAPK
signaling pathway.
MAPK activation. With aging, the response to PTH was significantly reduced. However,
The amount of basal protein expression determined by Western blot analysis for MAPK was not
different in the enterocytes from young and aged rats.
In conclusion, the results obtained in this work expand our knowledge on the mechanism of action
of PTH in duodenal cells, revealing that protein tyrosine phosphorylation is linked to the PTH
regulation of enterocyte MAPK activation, and that this mechanism is impaired with aging.
Understanding the molecular mechanisms for the age-related differences in PTH signaling will
require more information about the subtle mechanisms that modulate the PTH receptor-MAPK
signaling pathway.
(2 mM). Moreover, the tyrosine phosphorylation and activation of MAPK was dependent on Src
kinase, since PP1 (10 and 20 mM), a specific Src family tyrosine-kinase inhibitor, blocked PTHinduced
MAPK activation. With aging, the response to PTH was significantly reduced. However,
The amount of basal protein expression determined by Western blot analysis for MAPK was not
different in the enterocytes from young and aged rats.
In conclusion, the results obtained in this work expand our knowledge on the mechanism of action
of PTH in duodenal cells, revealing that protein tyrosine phosphorylation is linked to the PTH
regulation of enterocyte MAPK activation, and that this mechanism is impaired with aging.
Understanding the molecular mechanisms for the age-related differences in PTH signaling will
require more information about the subtle mechanisms that modulate the PTH receptor-MAPK
signaling pathway.
MAPK activation. With aging, the response to PTH was significantly reduced. However,
The amount of basal protein expression determined by Western blot analysis for MAPK was not
different in the enterocytes from young and aged rats.
In conclusion, the results obtained in this work expand our knowledge on the mechanism of action
of PTH in duodenal cells, revealing that protein tyrosine phosphorylation is linked to the PTH
regulation of enterocyte MAPK activation, and that this mechanism is impaired with aging.
Understanding the molecular mechanisms for the age-related differences in PTH signaling will
require more information about the subtle mechanisms that modulate the PTH receptor-MAPK
signaling pathway.
kinase, since PP1 (10 and 20 mM), a specific Src family tyrosine-kinase inhibitor, blocked PTHinduced
MAPK activation. With aging, the response to PTH was significantly reduced. However,
The amount of basal protein expression determined by Western blot analysis for MAPK was not
different in the enterocytes from young and aged rats.
In conclusion, the results obtained in this work expand our knowledge on the mechanism of action
of PTH in duodenal cells, revealing that protein tyrosine phosphorylation is linked to the PTH
regulation of enterocyte MAPK activation, and that this mechanism is impaired with aging.
Understanding the molecular mechanisms for the age-related differences in PTH signaling will
require more information about the subtle mechanisms that modulate the PTH receptor-MAPK
signaling pathway.
MAPK activation. With aging, the response to PTH was significantly reduced. However,
The amount of basal protein expression determined by Western blot analysis for MAPK was not
different in the enterocytes from young and aged rats.
In conclusion, the results obtained in this work expand our knowledge on the mechanism of action
of PTH in duodenal cells, revealing that protein tyrosine phosphorylation is linked to the PTH
regulation of enterocyte MAPK activation, and that this mechanism is impaired with aging.
Understanding the molecular mechanisms for the age-related differences in PTH signaling will
require more information about the subtle mechanisms that modulate the PTH receptor-MAPK
signaling pathway.
was effectively suppressed by the tyrosine-kinase inhibitors, genistein (100 mM) and herbimycin
(2 mM). Moreover, the tyrosine phosphorylation and activation of MAPK was dependent on Src
kinase, since PP1 (10 and 20 mM), a specific Src family tyrosine-kinase inhibitor, blocked PTHinduced
MAPK activation. With aging, the response to PTH was significantly reduced. However,
The amount of basal protein expression determined by Western blot analysis for MAPK was not
different in the enterocytes from young and aged rats.
In conclusion, the results obtained in this work expand our knowledge on the mechanism of action
of PTH in duodenal cells, revealing that protein tyrosine phosphorylation is linked to the PTH
regulation of enterocyte MAPK activation, and that this mechanism is impaired with aging.
Understanding the molecular mechanisms for the age-related differences in PTH signaling will
require more information about the subtle mechanisms that modulate the PTH receptor-MAPK
signaling pathway.
MAPK activation. With aging, the response to PTH was significantly reduced. However,
The amount of basal protein expression determined by Western blot analysis for MAPK was not
different in the enterocytes from young and aged rats.
In conclusion, the results obtained in this work expand our knowledge on the mechanism of action
of PTH in duodenal cells, revealing that protein tyrosine phosphorylation is linked to the PTH
regulation of enterocyte MAPK activation, and that this mechanism is impaired with aging.
Understanding the molecular mechanisms for the age-related differences in PTH signaling will
require more information about the subtle mechanisms that modulate the PTH receptor-MAPK
signaling pathway.
kinase, since PP1 (10 and 20 mM), a specific Src family tyrosine-kinase inhibitor, blocked PTHinduced
MAPK activation. With aging, the response to PTH was significantly reduced. However,
The amount of basal protein expression determined by Western blot analysis for MAPK was not
different in the enterocytes from young and aged rats.
In conclusion, the results obtained in this work expand our knowledge on the mechanism of action
of PTH in duodenal cells, revealing that protein tyrosine phosphorylation is linked to the PTH
regulation of enterocyte MAPK activation, and that this mechanism is impaired with aging.
Understanding the molecular mechanisms for the age-related differences in PTH signaling will
require more information about the subtle mechanisms that modulate the PTH receptor-MAPK
signaling pathway.
MAPK activation. With aging, the response to PTH was significantly reduced. However,
The amount of basal protein expression determined by Western blot analysis for MAPK was not
different in the enterocytes from young and aged rats.
In conclusion, the results obtained in this work expand our knowledge on the mechanism of action
of PTH in duodenal cells, revealing that protein tyrosine phosphorylation is linked to the PTH
regulation of enterocyte MAPK activation, and that this mechanism is impaired with aging.
Understanding the molecular mechanisms for the age-related differences in PTH signaling will
require more information about the subtle mechanisms that modulate the PTH receptor-MAPK
signaling pathway.
(2 mM). Moreover, the tyrosine phosphorylation and activation of MAPK was dependent on Src
kinase, since PP1 (10 and 20 mM), a specific Src family tyrosine-kinase inhibitor, blocked PTHinduced
MAPK activation. With aging, the response to PTH was significantly reduced. However,
The amount of basal protein expression determined by Western blot analysis for MAPK was not
different in the enterocytes from young and aged rats.
In conclusion, the results obtained in this work expand our knowledge on the mechanism of action
of PTH in duodenal cells, revealing that protein tyrosine phosphorylation is linked to the PTH
regulation of enterocyte MAPK activation, and that this mechanism is impaired with aging.
Understanding the molecular mechanisms for the age-related differences in PTH signaling will
require more information about the subtle mechanisms that modulate the PTH receptor-MAPK
signaling pathway.
MAPK activation. With aging, the response to PTH was significantly reduced. However,
The amount of basal protein expression determined by Western blot analysis for MAPK was not
different in the enterocytes from young and aged rats.
In conclusion, the results obtained in this work expand our knowledge on the mechanism of action
of PTH in duodenal cells, revealing that protein tyrosine phosphorylation is linked to the PTH
regulation of enterocyte MAPK activation, and that this mechanism is impaired with aging.
Understanding the molecular mechanisms for the age-related differences in PTH signaling will
require more information about the subtle mechanisms that modulate the PTH receptor-MAPK
signaling pathway.
kinase, since PP1 (10 and 20 mM), a specific Src family tyrosine-kinase inhibitor, blocked PTHinduced
MAPK activation. With aging, the response to PTH was significantly reduced. However,
The amount of basal protein expression determined by Western blot analysis for MAPK was not
different in the enterocytes from young and aged rats.
In conclusion, the results obtained in this work expand our knowledge on the mechanism of action
of PTH in duodenal cells, revealing that protein tyrosine phosphorylation is linked to the PTH
regulation of enterocyte MAPK activation, and that this mechanism is impaired with aging.
Understanding the molecular mechanisms for the age-related differences in PTH signaling will
require more information about the subtle mechanisms that modulate the PTH receptor-MAPK
signaling pathway.
MAPK activation. With aging, the response to PTH was significantly reduced. However,
The amount of basal protein expression determined by Western blot analysis for MAPK was not
different in the enterocytes from young and aged rats.
In conclusion, the results obtained in this work expand our knowledge on the mechanism of action
of PTH in duodenal cells, revealing that protein tyrosine phosphorylation is linked to the PTH
regulation of enterocyte MAPK activation, and that this mechanism is impaired with aging.
Understanding the molecular mechanisms for the age-related differences in PTH signaling will
require more information about the subtle mechanisms that modulate the PTH receptor-MAPK
signaling pathway.
M) with maximal stimulation achieved at 10-910-8 M. PTH-induced MAPK phosphorylation
was effectively suppressed by the tyrosine-kinase inhibitors, genistein (100 mM) and herbimycin
(2 mM). Moreover, the tyrosine phosphorylation and activation of MAPK was dependent on Src
kinase, since PP1 (10 and 20 mM), a specific Src family tyrosine-kinase inhibitor, blocked PTHinduced
MAPK activation. With aging, the response to PTH was significantly reduced. However,
The amount of basal protein expression determined by Western blot analysis for MAPK was not
different in the enterocytes from young and aged rats.
In conclusion, the results obtained in this work expand our knowledge on the mechanism of action
of PTH in duodenal cells, revealing that protein tyrosine phosphorylation is linked to the PTH
regulation of enterocyte MAPK activation, and that this mechanism is impaired with aging.
Understanding the molecular mechanisms for the age-related differences in PTH signaling will
require more information about the subtle mechanisms that modulate the PTH receptor-MAPK
signaling pathway.
MAPK activation. With aging, the response to PTH was significantly reduced. However,
The amount of basal protein expression determined by Western blot analysis for MAPK was not
different in the enterocytes from young and aged rats.
In conclusion, the results obtained in this work expand our knowledge on the mechanism of action
of PTH in duodenal cells, revealing that protein tyrosine phosphorylation is linked to the PTH
regulation of enterocyte MAPK activation, and that this mechanism is impaired with aging.
Understanding the molecular mechanisms for the age-related differences in PTH signaling will
require more information about the subtle mechanisms that modulate the PTH receptor-MAPK
signaling pathway.
kinase, since PP1 (10 and 20 mM), a specific Src family tyrosine-kinase inhibitor, blocked PTHinduced
MAPK activation. With aging, the response to PTH was significantly reduced. However,
The amount of basal protein expression determined by Western blot analysis for MAPK was not
different in the enterocytes from young and aged rats.
In conclusion, the results obtained in this work expand our knowledge on the mechanism of action
of PTH in duodenal cells, revealing that protein tyrosine phosphorylation is linked to the PTH
regulation of enterocyte MAPK activation, and that this mechanism is impaired with aging.
Understanding the molecular mechanisms for the age-related differences in PTH signaling will
require more information about the subtle mechanisms that modulate the PTH receptor-MAPK
signaling pathway.
MAPK activation. With aging, the response to PTH was significantly reduced. However,
The amount of basal protein expression determined by Western blot analysis for MAPK was not
different in the enterocytes from young and aged rats.
In conclusion, the results obtained in this work expand our knowledge on the mechanism of action
of PTH in duodenal cells, revealing that protein tyrosine phosphorylation is linked to the PTH
regulation of enterocyte MAPK activation, and that this mechanism is impaired with aging.
Understanding the molecular mechanisms for the age-related differences in PTH signaling will
require more information about the subtle mechanisms that modulate the PTH receptor-MAPK
signaling pathway.
(2 mM). Moreover, the tyrosine phosphorylation and activation of MAPK was dependent on Src
kinase, since PP1 (10 and 20 mM), a specific Src family tyrosine-kinase inhibitor, blocked PTHinduced
MAPK activation. With aging, the response to PTH was significantly reduced. However,
The amount of basal protein expression determined by Western blot analysis for MAPK was not
different in the enterocytes from young and aged rats.
In conclusion, the results obtained in this work expand our knowledge on the mechanism of action
of PTH in duodenal cells, revealing that protein tyrosine phosphorylation is linked to the PTH
regulation of enterocyte MAPK activation, and that this mechanism is impaired with aging.
Understanding the molecular mechanisms for the age-related differences in PTH signaling will
require more information about the subtle mechanisms that modulate the PTH receptor-MAPK
signaling pathway.
MAPK activation. With aging, the response to PTH was significantly reduced. However,
The amount of basal protein expression determined by Western blot analysis for MAPK was not
different in the enterocytes from young and aged rats.
In conclusion, the results obtained in this work expand our knowledge on the mechanism of action
of PTH in duodenal cells, revealing that protein tyrosine phosphorylation is linked to the PTH
regulation of enterocyte MAPK activation, and that this mechanism is impaired with aging.
Understanding the molecular mechanisms for the age-related differences in PTH signaling will
require more information about the subtle mechanisms that modulate the PTH receptor-MAPK
signaling pathway.
kinase, since PP1 (10 and 20 mM), a specific Src family tyrosine-kinase inhibitor, blocked PTHinduced
MAPK activation. With aging, the response to PTH was sig