INVESTIGADORES
FELITTI Silvina Andrea
artículos
Título:
The cytochrome c gene from the green alga Chlamydomonas reinhardtii. Structure and expression in wild-type cells and in obligate photoautotrophic (dk) mutants.
Autor/es:
FELITTI, S.A.; CHAN, R.L.; GONZÁLEZ SIERRA, M.; GONZÁLEZ, D.H.
Revista:
PLANT AND CELL PHYSIOLOGY
Editorial:
oxford journals
Referencias:
Año: 2000 vol. 41 p. 1149 - 1156
ISSN:
0032-0781
Resumen:
The expression of the Chlamydomonas reinhardtii cytochromeChlamydomonas reinhardtii cytochrome
c gene was studied at the steady-state mRNA level.
The inclusion of acetate under illumination produced a
marked increase in cytochrome c transcripts. This effect
was not affected by two inhibitors of mitochondrial energy
metabolism. Three different obligate photoautotrophic mutants
with defective mitochondria showed normal levels of
induction, suggesting that utilization of acetate for respiration
is not required for this process. Light, in the presence
or absence of acetate, also promoted an increase in cytochrome
was not affected by two inhibitors of mitochondrial energy
metabolism. Three different obligate photoautotrophic mutants
with defective mitochondria showed normal levels of
induction, suggesting that utilization of acetate for respiration
is not required for this process. Light, in the presence
or absence of acetate, also promoted an increase in cytochrome
was not affected by two inhibitors of mitochondrial energy
metabolism. Three different obligate photoautotrophic mutants
with defective mitochondria showed normal levels of
induction, suggesting that utilization of acetate for respiration
is not required for this process. Light, in the presence
or absence of acetate, also promoted an increase in cytochrome
was not affected by two inhibitors of mitochondrial energy
metabolism. Three different obligate photoautotrophic mutants
with defective mitochondria showed normal levels of
induction, suggesting that utilization of acetate for respiration
is not required for this process. Light, in the presence
or absence of acetate, also promoted an increase in cytochrome
was not affected by two inhibitors of mitochondrial energy
metabolism. Three different obligate photoautotrophic mutants
with defective mitochondria showed normal levels of
induction, suggesting that utilization of acetate for respiration
is not required for this process. Light, in the presence
or absence of acetate, also promoted an increase in cytochrome
The inclusion of acetate under illumination produced a
marked increase in cytochrome c transcripts. This effect
was not affected by two inhibitors of mitochondrial energy
metabolism. Three different obligate photoautotrophic mutants
with defective mitochondria showed normal levels of
induction, suggesting that utilization of acetate for respiration
is not required for this process. Light, in the presence
or absence of acetate, also promoted an increase in cytochrome
was not affected by two inhibitors of mitochondrial energy
metabolism. Three different obligate photoautotrophic mutants
with defective mitochondria showed normal levels of
induction, suggesting that utilization of acetate for respiration
is not required for this process. Light, in the presence
or absence of acetate, also promoted an increase in cytochrome
was not affected by two inhibitors of mitochondrial energy
metabolism. Three different obligate photoautotrophic mutants
with defective mitochondria showed normal levels of
induction, suggesting that utilization of acetate for respiration
is not required for this process. Light, in the presence
or absence of acetate, also promoted an increase in cytochrome
was not affected by two inhibitors of mitochondrial energy
metabolism. Three different obligate photoautotrophic mutants
with defective mitochondria showed normal levels of
induction, suggesting that utilization of acetate for respiration
is not required for this process. Light, in the presence
or absence of acetate, also promoted an increase in cytochrome
was not affected by two inhibitors of mitochondrial energy
metabolism. Three different obligate photoautotrophic mutants
with defective mitochondria showed normal levels of
induction, suggesting that utilization of acetate for respiration
is not required for this process. Light, in the presence
or absence of acetate, also promoted an increase in cytochrome
The inclusion of acetate under illumination produced a
marked increase in cytochrome c transcripts. This effect
was not affected by two inhibitors of mitochondrial energy
metabolism. Three different obligate photoautotrophic mutants
with defective mitochondria showed normal levels of
induction, suggesting that utilization of acetate for respiration
is not required for this process. Light, in the presence
or absence of acetate, also promoted an increase in cytochrome
was not affected by two inhibitors of mitochondrial energy
metabolism. Three different obligate photoautotrophic mutants
with defective mitochondria showed normal levels of
induction, suggesting that utilization of acetate for respiration
is not required for this process. Light, in the presence
or absence of acetate, also promoted an increase in cytochrome
was not affected by two inhibitors of mitochondrial energy
metabolism. Three different obligate photoautotrophic mutants
with defective mitochondria showed normal levels of
induction, suggesting that utilization of acetate for respiration
is not required for this process. Light, in the presence
or absence of acetate, also promoted an increase in cytochrome
was not affected by two inhibitors of mitochondrial energy
metabolism. Three different obligate photoautotrophic mutants
with defective mitochondria showed normal levels of
induction, suggesting that utilization of acetate for respiration
is not required for this process. Light, in the presence
or absence of acetate, also promoted an increase in cytochrome
was not affected by two inhibitors of mitochondrial energy
metabolism. Three different obligate photoautotrophic mutants
with defective mitochondria showed normal levels of
induction, suggesting that utilization of acetate for respiration
is not required for this process. Light, in the presence
or absence of acetate, also promoted an increase in cytochrome
The inclusion of acetate under illumination produced a
marked increase in cytochrome c transcripts. This effect
was not affected by two inhibitors of mitochondrial energy
metabolism. Three different obligate photoautotrophic mutants
with defective mitochondria showed normal levels of
induction, suggesting that utilization of acetate for respiration
is not required for this process. Light, in the presence
or absence of acetate, also promoted an increase in cytochrome
was not affected by two inhibitors of mitochondrial energy
metabolism. Three different obligate photoautotrophic mutants
with defective mitochondria showed normal levels of
induction, suggesting that utilization of acetate for respiration
is not required for this process. Light, in the presence
or absence of acetate, also promoted an increase in cytochrome
was not affected by two inhibitors of mitochondrial energy
metabolism. Three different obligate photoautotrophic mutants
with defective mitochondria showed normal levels of
induction, suggesting that utilization of acetate for respiration
is not required for this process. Light, in the presence
or absence of acetate, also promoted an increase in cytochrome
was not affected by two inhibitors of mitochondrial energy
metabolism. Three different obligate photoautotrophic mutants
with defective mitochondria showed normal levels of
induction, suggesting that utilization of acetate for respiration
is not required for this process. Light, in the presence
or absence of acetate, also promoted an increase in cytochrome
was not affected by two inhibitors of mitochondrial energy
metabolism. Three different obligate photoautotrophic mutants
with defective mitochondria showed normal levels of
induction, suggesting that utilization of acetate for respiration
is not required for this process. Light, in the presence
or absence of acetate, also promoted an increase in cytochrome
The inclusion of acetate under illumination produced a
marked increase in cytochrome c transcripts. This effect
was not affected by two inhibitors of mitochondrial energy
metabolism. Three different obligate photoautotrophic mutants
with defective mitochondria showed normal levels of
induction, suggesting that utilization of acetate for respiration
is not required for this process. Light, in the presence
or absence of acetate, also promoted an increase in cytochrome
was not affected by two inhibitors of mitochondrial energy
metabolism. Three different obligate photoautotrophic mutants
with defective mitochondria showed normal levels of
induction, suggesting that utilization of acetate for respiration
is not required for this process. Light, in the presence
or absence of acetate, also promoted an increase in cytochrome
was not affected by two inhibitors of mitochondrial energy
metabolism. Three different obligate photoautotrophic mutants
with defective mitochondria showed normal levels of
induction, suggesting that utilization of acetate for respiration
is not required for this process. Light, in the presence
or absence of acetate, also promoted an increase in cytochrome
was not affected by two inhibitors of mitochondrial energy
metabolism. Three different obligate photoautotrophic mutants
with defective mitochondria showed normal levels of
induction, suggesting that utilization of acetate for respiration
is not required for this process. Light, in the presence
or absence of acetate, also promoted an increase in cytochrome
was not affected by two inhibitors of mitochondrial energy
metabolism. Three different obligate photoautotrophic mutants
with defective mitochondria showed normal levels of
induction, suggesting that utilization of acetate for respiration
is not required for this process. Light, in the presence
or absence of acetate, also promoted an increase in cytochrome
gene was studied at the steady-state mRNA level.
The inclusion of acetate under illumination produced a
marked increase in cytochrome c transcripts. This effect
was not affected by two inhibitors of mitochondrial energy
metabolism. Three different obligate photoautotrophic mutants
with defective mitochondria showed normal levels of
induction, suggesting that utilization of acetate for respiration
is not required for this process. Light, in the presence
or absence of acetate, also promoted an increase in cytochrome
was not affected by two inhibitors of mitochondrial energy
metabolism. Three different obligate photoautotrophic mutants
with defective mitochondria showed normal levels of
induction, suggesting that utilization of acetate for respiration
is not required for this process. Light, in the presence
or absence of acetate, also promoted an increase in cytochrome
was not affected by two inhibitors of mitochondrial energy
metabolism. Three different obligate photoautotrophic mutants
with defective mitochondria showed normal levels of
induction, suggesting that utilization of acetate for respiration
is not required for this process. Light, in the presence
or absence of acetate, also promoted an increase in cytochrome
was not affected by two inhibitors of mitochondrial energy
metabolism. Three different obligate photoautotrophic mutants
with defective mitochondria showed normal levels of
induction, suggesting that utilization of acetate for respiration
is not required for this process. Light, in the presence
or absence of acetate, also promoted an increase in cytochrome
was not affected by two inhibitors of mitochondrial energy
metabolism. Three different obligate photoautotrophic mutants
with defective mitochondria showed normal levels of
induction, suggesting that utilization of acetate for respiration
is not required for this process. Light, in the presence
or absence of acetate, also promoted an increase in cytochrome
c transcripts. This effect
was not affected by two inhibitors of mitochondrial energy
metabolism. Three different obligate photoautotrophic mutants
with defective mitochondria showed normal levels of
induction, suggesting that utilization of acetate for respiration
is not required for this process. Light, in the presence
or absence of acetate, also promoted an increase in cytochrome
c transcript levels. This effect could be abolished by
treatment of the cells with an inhibitor of the photosynthetic
electron transport chain, suggesting that light acts
through photosynthesis to promote the induction. In addition,
a genomic clone encompassing the Chlamydomonas cytochrome
treatment of the cells with an inhibitor of the photosynthetic
electron transport chain, suggesting that light acts
through photosynthesis to promote the induction. In addition,
a genomic clone encompassing the Chlamydomonas cytochrome
treatment of the cells with an inhibitor of the photosynthetic
electron transport chain, suggesting that light acts
through photosynthesis to promote the induction. In addition,
a genomic clone encompassing the Chlamydomonas cytochrome
treatment of the cells with an inhibitor of the photosynthetic
electron transport chain, suggesting that light acts
through photosynthesis to promote the induction. In addition,
a genomic clone encompassing the Chlamydomonas cytochrome
treatment of the cells with an inhibitor of the photosynthetic
electron transport chain, suggesting that light acts
through photosynthesis to promote the induction. In addition,
a genomic clone encompassing the Chlamydomonas cytochrome
transcript levels. This effect could be abolished by
treatment of the cells with an inhibitor of the photosynthetic
electron transport chain, suggesting that light acts
through photosynthesis to promote the induction. In addition,
a genomic clone encompassing the Chlamydomonas cytochromeChlamydomonas cytochrome
c gene has been isolated and analyzed. The gene
contains three introns, two of which are located at positions
similar to those in the rice and Arabidopsis cytochrome
contains three introns, two of which are located at positions
similar to those in the rice and Arabidopsis cytochrome
contains three introns, two of which are located at positions
similar to those in the rice and Arabidopsis cytochrome
contains three introns, two of which are located at positions
similar to those in the rice and Arabidopsis cytochrome
contains three introns, two of which are located at positions
similar to those in the rice and Arabidopsis cytochrome
gene has been isolated and analyzed. The gene
contains three introns, two of which are located at positions
similar to those in the rice and Arabidopsis cytochromeArabidopsis cytochrome
c genes, indicating the existence of an evolutionary
link. It is concluded that the cytochrome c gene from C. reinhardtii
link. It is concluded that the cytochrome c gene from C. reinhardtii
link. It is concluded that the cytochrome c gene from C. reinhardtii
link. It is concluded that the cytochrome c gene from C. reinhardtii
link. It is concluded that the cytochrome c gene from C. reinhardtii
genes, indicating the existence of an evolutionary
link. It is concluded that the cytochrome c gene from C. reinhardtiic gene from C. reinhardtii
is subject to metabolic regulation through a mechanism
that responds to the intracellular level of either acetate
or a compound derived from its metabolization
through a pathway different from mitochondrial respiration.