INVESTIGADORES
FELITTI Silvina Andrea
artículos
Título:
Genome polymorphisms and gene differential expression in a back-and-forth ploidy-altered series of weeping lovegrass (Eragrostis curvula)
Autor/es:
MECCHIA, M.A.; OCHOGAVÍA, A.; SELVA, J.P.; LASPINA, N.; FELITTI, S.A.; MARTELOTTO, L.G.; SPANGENBERG, G.C.; ECHENIQUE, V.; PESSINO, S.C.
Revista:
JOURNAL OF PLANT PHYSIOLOGY
Editorial:
Elsevier
Referencias:
Lugar: Alemania; Año: 2007 vol. 164 p. 1051 - 1061
ISSN:
0176-1617
Resumen:
Molecular markers were used to analyze the genomic structure of an euploid series
of Eragrostis curvula, obtained after a tetraploid dihaploidization procedure
followed by chromosome re-doubling with colchicine. Considerable levels of genome
polymorphisms were detected between lines. Curiously, a significant number of
molecular markers showed a revertant behavior following the successive changes of
ploidy, suggesting that genome alterations were specific and conferred genetic
structures characteristic of a given ploidy level. Genuine reversion was confirmed by
sequencing. Cluster analysis demonstrated grouping of tetraploids while the diploid
was more distantly related with respect to the rest of the plants. Polymorphic
revertant sequences involved mostly non-coding regions, although some of them
displayed sequence homology to known genes. A revertant sequence corresponding
to a P-type adenosine triphosphatase was found to be differentially represented in
cDNA libraries obtained from the diploid and a colchiploid, but was not found
expressed in the original tetraploid. Transcriptome profiling of inflorescence
followed by real-time polymerase chain reaction validation showed 0.34%
polymorphic bands between apomictic tetraploid and sexual diploid plants. Several
of the polymorphic sequences corresponded to known genes. Possible correlation
between the results observed here and a recently reported genome-wide non-
Mendelian inheritance mechanism in Arabidopsis thaliana are discussed.