Analysis of adenocarcinoma of colon and rectum: detection of human papillomavirus (HPV) DNA by polymerase chain reaction

PÉREZ LO; ABBA MC; LAGUENS RM; GOLIJOW CD

COLORECTAL DISEASE (PRINT)

Blackwell Publishing Ltd.

Año: 2005 vol. 7 p. 492 - 495

1462-8910

Objective The aim of the present work was to evaluate the presence of human papillomavirus genotypes in malignant and normal mucosa of the colon and rectum in order to determine if a relationship exists between HPV infection and colon neoplasms. Materials and methods Thirty normal colon tissues and 54 sporadic adenocarcinomas were screened for HPV positivity using nested-PCR. Detection of viral types 6, 11, 16, 18, 33, 34 and 51 was performed by the LISSSCP (Low Ionic Strength-Single Strand Conformational Polymorphism) procedure.Results Significant differences in high risk HPV infection were found between normal samples and adenocarcinomas (P < 0.001). Among the cases, an inverse association between HPV infection and Dukes staging was also found (P = 0.020). Finally, there was no significant association between HPV and some classical clinicopathological features, although a gradient ofinfection form rectum to cecum was evident. Conclusion The present study demonstrates that HPV may infect the glandular mucosa of the colon and suggests a possible association between HPV and colorectal cancer.