Producción de anticuerpos monoclonales específicos para la proteína KIF4

LUJAN HUGO; ROPOLO ANDREA; SAURA ALICIA; CACERES ALFREDO

2006-03-10

2006-07-25

Biológica

Otros campos

  BACKGROUND The kinesins constitute a large family of microtubule-dependent motor proteins, which are responsible for the distribution of numerous organelles, vesicles and macromolecular complexes throughout the cell. Individual kinesin members play crucial roles in cell division, intracellular transport and membrane trafficking events. KIF4 is a microtubule plus end-directed anterograde motor composed of an NH2-terminal globular motor domain, a central a-helical stalk domain, and a COOH-terminal tail domain. KIF4 forms a homodimer that moves along microtubules towards the plus-end at a velocity of 0.2 mm/s. It is expressed abundantly in juvenile tissues, including differentiated young neurons; in adult mice, its expression is considerably decreased, except in the spleen. KIF4 is colocalized with membranous organelles in the growth cones of differentiated neurons as well as in the cytoplasm and nucleus of cultured fibroblasts. Evidence has been shown of KIF4 transporting membranous organelles containing L1, a cell adhesion molecule implicated in axonal elongation in juvenile neurons. During the mitotic phase of the cell cycle, KIF4 appears to colocalize with membranous organelles in the mitotic spindle and it also been implicated in an essential role in midzone formation and in the cytokinesis.  SOURCE The mouse monoclonal antibody 3E2 (IgG) was raised using as immunogen the last 161 aminoacids of the COOH-terminal tail domain of rat KIF4. APPLICATION APPLICATION APPLICATION APPLICATION The mAb 3E2 recognizes a single band of 140 kDa corresponding to KIF4. It does not cross react with other kinesin-related proteins, such as KIF5 or KIF2. The mAb 3E2 is recommended for detection of KIF4 of rat origin by Western Blotting (starting dilution 1:250), immunofluorescence (starting dilution 1:100) and immunoprecipitation.  SOURCE The mouse monoclonal antibody 3E2 (IgG) was raised using as immunogen the last 161 aminoacids of the COOH-terminal tail domain of rat KIF4. APPLICATION APPLICATION APPLICATION APPLICATION The mAb 3E2 recognizes a single band of 140 kDa corresponding to KIF4. It does not cross react with other kinesin-related proteins, such as KIF5 or KIF2. The mAb 3E2 is recommended for detection of KIF4 of rat origin by Western Blotting (starting dilution 1:250), immunofluorescence (starting dilution 1:100) and immunoprecipitation.  SOURCE The mouse monoclonal antibody 3E2 (IgG) was raised using as immunogen the last 161 aminoacids of the COOH-terminal tail domain of rat KIF4. APPLICATION APPLICATION APPLICATION APPLICATION The mAb 3E2 recognizes a single band of 140 kDa corresponding to KIF4. It does not cross react with other kinesin-related proteins, such as KIF5 or KIF2. The mAb 3E2 is recommended for detection of KIF4 of rat origin by Western Blotting (starting dilution 1:250), immunofluorescence (starting dilution 1:100) and immunoprecipitation.