P300 involvement in apoptosis and metastasis in triple negative breast carcinoma (TNBC).

GALLARDO GA; CLEMENTE V.; IBARRA A.; FACCHINETTI M.M.; CURINO A. C.; FERMENTO M.E.

Mar del Plata

Congreso; LXV REUNIÓN ANUAL DE LA SOCIEDAD ARGENTINA DE INVESTIGACIÓN CLÍNICA (SAIC); 2020

SAIC

Triple-negative breast cancer (TNBC) is a heterogeneous group oftumors that lack specific molecular targets. Therefore, it is necessary to investigate potential tumor markers for this subtype of BC.There are few studies in TNBC on the role of p300 in apoptosisand they are contradictory. Previously, we published that pharmacological inhibition of p300 increases apoptosis in murine BC cells,however, it is necessary to check whether the same effect occurs inhuman TNBC cells. Recently a prometastatic role of p300 in BC hasbeen found. In addition, we demonstrated that pharmacological inhibition of p300 decreases migration and adhesion in TNBC, showingthe need to delve into the molecules involved. Hence, the aim of thiswork was to study the effect of the inhibition of the acetylase function of p300 in the processes of apoptosis and metastasis in humanTNBC cells. The MDA-MB-231 cell line was treated with VV59 (inhibitor of the acetylase function of p300) or its vehicle (DMSO). In acell viability assay, we observed that the pharmacological inhibitionof p300 produced a decrease in the number of cells compared tovehicle (p