Macrophage?s role in the microenvironment against Epstein Barr Virus (EBV) in tonsils from pediatrics patients

MOYANO A; FERRESSINI GERPE, NM; DE MATTEO E; PRECIADO MV; PAOLA ANDREA CHABAY

Congreso; Reunión Anual Sociedades de Biociencia. SAIC. SAI. SAFIS. 2020; 2020

SAIC

In Argentina, a significant association of EBV with pediatric lymphomas was described. Little is known about macrophage response in EBV infection. Our aim was to study the macrophage´s role in pediatric EBV infection to help clarify viral contribution tolymphomagenesisWe studied 74 patients undergoing tonsil surgery. The infection status was assessedby Anti-EBV VCA-IgM, VCA-IgG, EA-IgG and EBNA1-IgGindirect immunofluorescence, defining 4 status: primary infection (PI), healthy carrier (HC), reactivation (R) and not infected (NI). Viral type and load, IL-1 and TNF-α were assessed by PCR and qPCR. Latency pattern was evaluated by immunohistochemistry (IHC) for LMP1, EBNA2 and BMRF1, and by EBERS in situ hybridization (ISH). Macrophage´s characterization was performed by CD68, CD163, and CD169 IHC, in germinal center (GC) and interfollicular (IF) regions, expressed as positive cells/mm2. M1 profile was defined as CD68/CD163 >1.5 and M2 as CD163/CD68 >1.5Fifty-two percent of the patients were HC, 27,0% PI, 14,9% R and 6.8% were NI; 41.5% expressed Latency I, 31.7% Latency II, 12.2% Latency III, 14.6% Latency 0 and 14.6% also expressed lytic antigens; 89% of the patients displayed M1 profile. CD68+ cell counts was higher than CD163+ and CD169+ in the entire series and within groups (ANOVA p