Characterization of macrophage innate immune response at the site of EBV entry in pediatric patients

MOYANO A; FERRESSINI N; VISTAROP A; CALDIROLA MS; GAILLARD, M. I.; DE MATTEO E; PRECIADO, M. V.; PAOLA ANDREA CHABAY

Trieste

Congreso; ICGEB DNA Tumour Virus Meeting ? 50th Anniversary; 2019

ICGEB

Macrophage?s role in the management of Epstein Barr virus (EBV) infection is unexplored. Therefore, our aim is to characterize macrophages in tonsil microenvironment in pediatric patients infected with EBV. Methods: We studied 72 patients with tonsil surgery. The infection status was studied by Anti-EBV VCA-IgM, VCA-IgG, EA-IgG and -EBNA1 IgG, to define primary infection (PI), healthy carrier (HC), reactivation (R) and no infected (NI). Viral load and typification was assessed by PCR. Latency pattern was evaluated by Immunohistochemistry (IHC) for LMP1, EBNA2 and BMRF1, and EBERS in Situ Hybridization (ISH). Macrophages characterization was performed by CD68, CD163, and CD169 IHC,in germinal center (GC) and interfollicular (IF) regions and results were expressed as positive cells/mm2.Results:Of our patients, 38 wereHC, 20 PI, 10 had viral Rand 4 were NI. 41.5% of patients expressed Latency I, 31.7% Latency II, 12.2% Latency III and 14.6% Latency 0, while 14.6% also showed positive cells for lyticantigens. We defined the macrophage´spolarization profile M1as CD68/CD163 >1.5, and M2 as CD163/CD68 >1.5;M1 profile was observed in 89% of the patients. CD68+ cell counts was statically higher compared to CD163+ and CD169+ in the entire series and within groups (ANOVA p