INVESTIGADORES
GARCIA Cybele Carina
congresos y reuniones científicas
Título:
Activity of Zn finger inhibitors against the arenavirus Junín
Autor/es:
E. B. DAMONTE; C. C. GARCÍA; NÉLIDA A. CANDURRA
Lugar:
Baltimore, USA
Reunión:
Conferencia; Thirteenth International Conference on Antiviral Research; 2000
Resumen:
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¨
Junin virus (JUNV), a
member of Arenaviridae, is the etiological agent of Argentine hemorrhagic
fever, an endemo-epidemic disease affecting population of the most fertile
farming land of Argentina. Current specific therapy consists of early administration
of immune plasma. Ribavirin is the only compound that has shown partial efficacy against JUNV
infections but with a high level of undesirable secondary reactions.
¨
JUNV is an enveloped virus
containing a bisegmented ambisense RNA genome. Only five proteins are expressed
from the two genome fragments; the nucleocapsid protein (NP), two envelope
glycoproteins (GP1 and GP2), an RNA polymerase (L) and a 11-kD protein (Z or
p11) with unknown function. The Z protein contains a RING finger motif with
cysteines and histidine to bind Zn. The conservation of this domain in
arenaviruses turns this protein an attractive target for antiviral therapy.
¨
In the present study,
several compounds with known reactivity toward the retroviral Zn finger motifs
of the HIV-1 p7 nucleocapsid protein were evaluated for their capacity to act
as arenavirus inhibitors. Different classes of electrophilic reagents with
diverse chemical structures, provided by the National Cancer Institute
(Frederick, USA) were tested against JUNV, Tacaribe virus(TACV) and Pichinde
virus (PICV).
