Immunocytochemical localization of different cell types in the adenohypophysis of the cichlid fish Cichlasoma dimerus (Heckel, 1840).

M. PANDOLFI; D. A. PAZ; M. C. MAGGESE; F. J. MEIJIDE; P. G. VISSIO

BIOCELL

Scielo

Lugar: Mendoza; Año: 2001 vol. 25 p. 35 - 42

0327-9545

The adenohypophysis (ADH) of the cichlid fish Cichlasoma dimerus was studied using the avidin-biotin-peroxidase method with antisera raised against piscine pituitary hormones and heterologous antisera against mammalian pituitary hormones. Antisera raised against rabbit ACTH recognized a group of cells bordering the neurohypophysis (NH) in the rostral pars distalis (RPD). Anti-chum salmon prolactin (PRL) identified a compact groups of cells in the periphery of the RPD. Gonadotropin (GTH II), thyrotropin (TSH) and growth hormone (GH)-ir cells were localized in the proximal pars distalis. Ir-GTH II cells were also observed in the dorsal area of the pars intermedia (PI). Ir-GTH I cells could not be identified using anti-chum salmon GTH I and that may be due to a failure of antisera to recognize the gonadotropin or to low expression of the hormone in adults of this species. PAS positive cells from the pars intermedia bound specifically with three different antisera raised against somatolactin (SL) of four different fish species. These cells sorrounded deep branches of the NH in the PI.