Effects of thyroid hormones on oxidative parameters in murine lymphocytic cells.

MACRI DELBONO, R; COSTILLA, M.; KLECHA, A. J; CREMASCHI, G.; BARREIRO ARCOS, M.L.

Buenos Aires

Congreso; Reunión Conjunta de Sociedades de Biociencias; 2017

Sociedad Argentina de Investigación Clínica (SAIC) en conjunto con Sociedades de Biociencias

BACKGROUND: Thyroid hormones exert actions on growth, metabolism, cellular differentiation and immune functionality. OBJECTIVE: To study the effects of hyperthyroidism on oxidative parameters in murine lymphocytes. METHODS: Female BALB/c mice were treated with T4 (12mg/l) in drinking water for 30 days (hyperthyroid animals) or with placebo (euthyroid animals). Serum hormone levels were quantified by radioimmunoassay. Reactive oxygen species (ROS) and apoptosis were evaluated by flow cytometry using DCFH-DA and Annexin-V/PI. The expression of antioxidant enzymes and iNOS were analyzed by real-time PCR and western blot. Enzymatic activity was quantified by spectrophotometry. Nitrite production was evaluated by the Griess technique. RESULTS: We observed an increase of ROS in lymphocytes purified from lymph node tissue (LN) and spleen (S) of hyperthyroid animals with respect to controls (29.1±2.6 % in LN and 26.2±2.1 in S, p