Mechanism of the antiproliferative action of limonene on a lymphoma cell line: participation of nitric oxide antiproliferative action of limonene on a lymphoma cell line

MANUELE M.G.; BARREIRO ARCOS M.L.; DAVICINO R.; FERRARO G.; CREMASCHI G.A.; ANESINI C.

PHYTOTHERAPY RESEARCH

JOHN WILEY & SONS LTD

Año: 2009 vol. 23 p. 1011 - 1017

0951-418X

Previously limonene demonstrated antiproliferative action on a lymphoma cell line (BW5147). The objective of this study was to investigate the mechanism of the antiproliferative action of limonene on BW 5147 cells. The induction of apoptosis and arrest in cell cycle phases, both phenomena related to nitric oxide (NO) production, was analyzed, as well as NO production. Results showed that limonene exhibited antiproliferative action on tumoral lymphocytes exerting a decrease in cell viability that was related to apoptosis induction and to the increase in NO levels at long incubation times. At short times and depending on its concentration, limonene arrested cells in different phases of the cell cycle, related to NO production.