INVESTIGADORES
BECERRA Alejandra Gabriela
congresos y reuniones científicas
Título:
Biocontrol of the phytophagous nematode Nacobbus aberrans by Pseudomonas fluorescens strain CHA0
Autor/es:
LAX, P., BECERRA, A., MARRO, N., AGARAS B, VALVERDE C. AND DOUCET, M.
Reunión:
Workshop; PGPR 9th International and 1st Latinamerican PGPR Workshop; 2012
Resumen:
Nacobbus aberrans is a sedentary
endoparasite that induces galls in root systems. It has a high reproductive
potential and it causes severe economical damage to different horticultural
crops. In Argentina,
this nematode affects greenhouse- and field-grown tomato. Several Pseudomonas strains produce extracellular secondary metabolites that
are toxic for phytopathogenic fungi, certain protists and phytophagous
nematodes, and are able to induce systemic resistance in plants. Strain ARQ1 is an isogenic gfp/KmR-tagged derivative from the biocontrol isolate P. fluorescens CHA0. We studied the
effect of ARQ1 (BACT) on a N. aberrans
population (NEM) from Lisandro Olmos (Buenos
Aires, Argentina)
in tomato seedlings (cv.
Platense) grown in sterilized soil under greenhouse conditions. Four treatments (5 replicates) were set
up: a) Control; b) BACT, c) NEM, d) NEM+BACT. The inocula consisted in
100 second-stage juveniles and/or 10 ml of a suspension of ARQ1 cells at OD600
= 0.1. At 60 days after inoculation (DAI), the number of galls (G) and egg masses
(EM) produced by the nematode were determined. We observed a significant reduction (P<0.05) in both
parameters in NEM+BACT plants (G=20; EM=11) compared to NEM plants (G=34;
EM=19). At 15 DAI, the roots of NEM+BACT plants were colonized by ARQ1 cells at
1.0 ± 0.02 ´106
UFC/g whereas at 60 DAI, roots contained 3.0 ± 0.7 ´104 UFC/g. These
results show that P. fluorescens
strain CHA0 colonized tomato roots and had an antagonistic effect on the N. aberrans population.