The effect of dwarfing genes on the rhizosphere bacterial community colonization under limiting phosphorus conditions

FIGUEROA GALVIS, INGRID PAOLA; GUALANO, LEONARDO; MORICONI, JORGE IGNACIO; SANNAZZARO, ANALIA INÉS; SANTA-MARÍA, GUILLERMO ESTEBAN

Congreso; XXXIV Reunión de la Sociedad Argentina de Fisiología Vegetal; 2023

The modification of components of the Gibberellin-Gibberellin Receptor-DELLAs module (GA-GID-DELLAs) was pivotal for the green revolution. Current evidence indicates that it contributes to determine nutrient acquisition, while also influences key symbiotic interactions. The extent to what it globally influences plant soil microbe interactions awaits to be explored. Here we inform the colonization patterns of soil bacterial communities in the rhizosphere of barley (Hordeum vulgare) near isogenic lines affecting some components of the GA-GID-DELLAs module, grown under phosphorus limiting conditions. Plants of WT, M463 (defective in GAs synthesis) and M640 (dwarf, bearing an altered DELLA allele) lines were grown in a low phosphorus (P) soil. The rhizosphere bacterial community was assessed by high throughput 16S rRNA gene sequencing. Alpha and beta diversities were calculated to identify diversity within and between samples respectively. Different abundance tests were performed to find significant taxa associated with the barley lines. 5,114 OTUs sharing 97% similarity were identified. The most abundant taxa at phylum level were Proteobacteria (37.2-54.7%), Actinobacteria (11.5-32.9%) and Acidobacteria (8.0-18.2%). The most abundant genera were Sphingomonas, Massilia and Gaiella. Alpha and beta diversities of the bacterial community were not significantly different among the three barley lines. However, between the three genotypes, abundances of 2 phyla, 1 class, 2 orders, 3 families and 3 genera were found significantly different (P