Amperometric Biosensor for Phenols and Catechols Based on Iridium-Polyphenol Oxidase Modified Carbon Paste

M. D. RUBIANES AND G. A. RIVAS

ELECTROANALYSIS

Wiley-VCH Verlang GmbH & Co, KgaA, Weinheim

Lugar: Las Cruces, New México State, EEUU; Año: 2000 vol. 12 p. 1159 - 1162

1040-0397

An amperometric bioelectrode for the quantification of phenols and catechols based on the incorporation of iridium microparticles and poliphenol oxidase (PPO) within carbon paste matrices, is described. Iridium-doped carbon paste electrode displays excellent electrocatalytic activity towards the reduction of the enzymatically formed quinone species. Thus, the quantification of the micromolar/submicromolar levels of phenols and catechols can be performed at potentials lower than in the case of bare CPE with much better sensitivity (around 4-fold higher). The analitycal performance as well as the kinetics parameters for different substrates of clinical, pharmacological and environmental interest, are discussed.