INVESTIGADORES
NUSBLAT Alejandro David
artículos
Título:
A bioreactor model system specifically designed for Tetrahymena growth and cholesterol removal from milk
Autor/es:
NOSEDA DG; GENTILI HG; NANI ML; NUSBLAT A; TIEDTKE A; FLORIN-CHRISTENSEN J; NUDEL CB
Revista:
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
Editorial:
Springer Verlag
Referencias:
Lugar: Alemania; Año: 2007 vol. 75 p. 515 - 520
ISSN:
0175-7598
Resumen:
This work describes the configuration and operation of a bioreactor
system especially designed for Tetrahymena cultivation and its use for
milk improvement, particularly cholesterol elimination by the action of
this cell. An advantage of the proposed method is the re-use of the
growth medium; thus, the medium is used twice to provide two batches of
Tetrahymena biomass without the need of further inoculation. This makes
the procedure of producing the cell biomass faster and more economical.
Cells are concentrated in the culture vessels by sedimentation at room
temperature and then transferred to milk suspensions, where they can
further grow for at least one generation with the benefit of reducing
steeply cholesterol level. Milk treated according to this process is
separated from the biomass by centrifugation. Under these conditions,
less than 5% of the cells remain in the milk, and cholesterol
elimination amounts to 75 +/- 10% of that initially present. No changes
in sensorial properties of the milk, such as clotting or butyric odor,
were observed as a result of this treatment. In addition, the
bioreactor allows the aseptic recovery of the spent growth medium,
which contains diverse enzymes of interest, and the cell pellets, to
exploit particular lipids like phosphonolipids, abundant
poly-unsaturated fatty acids and co-enzyme Q(8).