NORADRENERGIC NEUROTRANSMITION IN CENTRAL NERVIOUS SYSTEM IN C57BL/6 MICE INFECTED WITH Trypanosoma cruzi

QUAGLIA N, POLLACHINI N, PEREZ AR, WILDMANN J, BESEDOVSKY H, BOTTASSO O, DEL REY A, ROGGERO E

Rosario, Argentina

Otro; XX Annual Meeting of the Argentine Society of Protozoology; 2004

Sociedad Argentina de Protozoología

Biocell Vol 28 Nº3 – 2004 XX Annual Meeting of the Argentine Society of Protozoology, May 26-28, 2004 NORADRENERGIC NEUROTRANSMITION IN CENTRAL NERVIOUS SYSTEM IN C57BL/6 MICE INFECTED WITH Trypanosoma cruzi NORADRENERGIC NEUROTRANSMITION IN CENTRAL NERVIOUS SYSTEM IN C57BL/6 MICE INFECTED WITH Trypanosoma cruzi Quaglia N1, Pollachini N1, Perez AR1, Wildmann J2, Besedovsky H2,Bottasso O1, del Rey A2, Roggero E1. 1Inst. de Inmunología, Fac. Cs. Médicas UNR. 2Inst. de FisiologíaNormal y Patológica, Universidad Philipps, Alemania. E-mail: earoggero@.yahoo.com.ar 1Inst. de Inmunología, Fac. Cs. Médicas UNR. 2Inst. de FisiologíaNormal y Patológica, Universidad Philipps, Alemania. E-mail: earoggero@.yahoo.com.ar 1Inst. de Inmunología, Fac. Cs. Médicas UNR. 2Inst. de FisiologíaNormal y Patológica, Universidad Philipps, Alemania. E-mail: earoggero@.yahoo.com.ar 1Inst. de Inmunología, Fac. Cs. Médicas UNR. 2Inst. de FisiologíaNormal y Patológica, Universidad Philipps, Alemania. E-mail: earoggero@.yahoo.com.ar It is known that the immuno-endocrine and neural systems are profoundly interrelated. The aim of this study was to analyze the noradrenaline (NA) content in the central nervous system (CNS) tissues and their possible relationship with some immuno-endocrine parameters during acute Trypanosoma cruzi infection in C57BL/6 mice. The analysis focused on plasma levels of pro-inflammatory cytokines (IL-1b, IL-6 and FNTa; ELISA, pg/ml); corticosterone (CT; RIA, ug/dl); as well as NA and their metabolite 3-metoxi,4- hidroxifeniletilenglicol (MHPG) (HPLC, ng/g tissue) in brain stem (BS), hypothalamus (Ht) e hippocampus (Hc). Results: (mean±sem, n=4-7; *=p<0,05) IL-1b Control (Co): nd, Tc: 89.5±6*; IL-6 Co: nd, Tc 163 ± 85*; FNTa Co: 13±1, Tc: 357 ± 42*; CT Co: 2.14±1.24; Tc: 9.3±1.77*; NA (BS) Co: 716±10, Tc 640±22*; NA (Ht) Co: 1500±118, Tc: 1370±58; NA (Hc) Co: 399±47, Tc: 295±26*. NA utilization was valuated as MHPG/NA ratio in BS Co: 0.076±0.009, Tc: 0.060±0.004*; Ht Co: 0.048±0.004, Tc: 0.058±0.006; and Hc Co: 0.054±0.008, Tc: 0.048±0.006. Conclusion: infected mice showed significantly increased levels of pro-inflammatory cytokines and CT, in presence of a reduced NA content and utilization in BS, with a diminished NA content being only found in Hc. There were no between group differences in NA content or utilization in Ht.