Sexual dimorphism of cellular and humoral immunity in experimental vaccines against Trypanosoma cruzi

BULFONI BALBI C.; PACINI MF ; DINATALE B ; GONZÁLEZ F; PROCHETTO E; FARRE C.; CHAPO. G; MARCIPAR I.; CABRERA G.,; PEREZ AR.

Mar del Plata

Congreso; Congreso Sociedades Biociencias 2021; 2021

Sociedad Argentina de Inmunología

(Pag 130 Libro de resúmenes)Resumen 202. There is evidence that vaccine efficacy is affected by diverse parameters like sex between others. Thus, the inclusion of both sexes in studies of immune function becomes increasingly imperative. Usually, vaccine research against T. cruzi (Tc) has been focused on antibody response evaluation. Thus, we aim to evaluate possible variations in humoral and cellular response induced after experimental vaccine administration against Tc between BALB/c males (M) and females (F). Thus, mice (n=5/group) were immunized intranasally [3 doses, one every 2 weeks with 10µg of TS emulsified in ISPA (I) or c-di-AMP (A) adjuvants]. Controls were treated with saline (S) or TS alone. Fifteen days after the last immunization, in vivo cell-mediated (delayed hypersensitivity test -DHT-) and specific humoral (ELISA) responses were assayed. Later, mice were orally challenged with 3000 Tc (sub-lethal challenge). Parasitemia and clinical score were evaluated until day 100 post-infection (pi). In both M and F, the DHT was higher in TS+I and TS+A groups compared to their respective controls (p