INVOLVEMENT OF NAD-Poly (ADP-Ribose) METABOLISM ON P53 REGULATION BY TOPOTECAN.

E. I. YSLAS, P.A. VALDECANTOS, M. ROLDAN-OLARTE AND C. MERCHED WHITACRE

Florida Miami

Workshop; en Academy of Sciences 67th; 2003

We have evaluated the effect of  NAD/Poly (ADP-Ribose) polymerase (PARP) metabolism deficiency on Topotecan induced apoptosis in Hela cervical cancer cells. Cells grown in the absence or presence of NAD were treated during 24-48 hs with increasing doses Tpotecan (0.1-1μM). Our studies suggest that NAD/ PARPsynthesis may be involved in TPT-induced apoptotic pathway and p53 regulation.