BACTERIAL INACTIVATION BY THE EFFECT OF NANOCOMPOSITE BASED ON ZINC OXIDE NANOPARTICLES

JESICA YANINA PEREYRA; DIEGO F. ACEVEDO; EDITH INES YSLAS; CESAR ALFREDO BARBERO

cancun

Congreso; XXVII International Materials Research Congress - IMRC 2018; 2018

In this work, the synthesis, characterization and application as bactericidal material of thenanocomposite is shown. The hydrogel was synthesized by copolymerization betweenN-isopropylacrylamide (NIPAM) and hydroxiethylmetha-acrylate (HEMA) (PNIPAM-co-10%HEMA). Zinc oxide nanoparticles (NPsZnO) were synthesized insitu inside of thebiocompatible hydrogel the precursor salt zinc nitrate. The zinc nitrate was allowed todiffuse inside the hydrogel for 24 hours. Then the swelling hydrogel was immersed inKOH solution and then the nanoparticles were obtained by using microwave. Theantimicrobial activity of the nanocomposite was evaluated using a Gram negative (-)bacterium as the Pseudomonas aeruginosa model. This action was studied by means of the diffusionmethod in agar, which was evidenced by the formation of inhibition halos. The size of the inhibitionhalos increases whit the incubation time (24-48 h) suggesting an important antibacterial effect of thenanocomposite. From the results of viability and inhibition halos using as a control group the hydrogeland as a group treated the nanocomposite at different incubation times a marked antibacterial actionof the Zinc oxide nanocomposite (PNIPAM-co-10% HEMA) dependent on time was observed. Inconclusion, these results reveal that zinc oxide generated inside of PNIPAM-co-10%HEMA can be usedas intelligent hydrogel to kill bacteria