DIFFERENT KINDS OF POLYMERIC NANOPARTICLES WITH APPLICATION IN ANTIBACTERIAL PHOTOTHERMAL THERAPY

SILVESTRE BONGIOVANNI ABEL ; MARTA DARDANELLI; CESAR BARBERO; CLAUDIA RIVAROLA; YSLAS * E.I.

Mar del Palta

Congreso; Reunión Conjunta de la Sociedad Argentina de Investigación Clínica (SAIC), la Sociedad Argentina de Inmunología (SAI) y la Sociedad Argentina de Fisiología (SAFIS) se suman la Sociedad Argentina de Virología (SAV), la de Nanomedicina; 2018

Sociedad Argentina de Investigación Clínica (SAIC), la Sociedad Argentina de Inmunología (SAI) y la Sociedad Argentina de Fisiología (SAFIS) se suman la Sociedad Argentina de Virología (SAV), la de Nanomedicina

Due to the high resistance to antibiotics developed by different bacteria, new methods are currently required for the treatment of infections generated by these pathogens. Among the alternative therapeutic modalities to the conventional ones, photothermal therapy(PTT) stands out. It is based on the absorption of radiation (e.g.NIR light) by molecules or materials and the subsequent generationof heat in the surrounding medium when transforming the incidentenergy. This phenomenon can be exploited to cause the death of thepathogen. (1) In this sense, with the rise of nanotechnology, differentmaterials have been synthesized and characterized to be applied inPTT. In this work, we demonstrate how is possible to use nanoparticles based on conducting polymers (NP-PANI) generated by different methods (oxidative polymerization from monomer (2) andsolvent displacement -nanoprecipitation?(3)) to decrease via PTT the viability of pathogenic bacteria such as Pseudomonas aeruginosa and Staphylococcus aureus, both responsible for a large number of nosocomial infections. The results show that in both casesmore than 80% of the viability is reduced, regardless of the kind ofnanoparticle, there being an antibacterial synergistic effect given bythe contribution of the nanoparticulate conducting polymer and theaction of the NIR light. The PTT phenomenon was corroborated bytechniques such as florescence microscopy and DNA fragmentation. (1). References:1. S. Bongiovanni Abel et al. Biomed. Phys.Eng. Express 4, 2018, 045037.2. S. Bongiovanni Abel et al. Nanotechnology 25(49), 2014,3.S.BongiovanniAbel et al. Nanotechnology 29, 2018, 125604.