Hybrid Biomaterials Based on Calcium Carbonate and Polyaniline Nanoparticles for Application in Photothermal Therapy

ANDRONICO NEIRA-CARRILLO ; EDITH YSLAS; YAZMIN AMAR MARINI ; PATRICIO VASQUEZ-QUITRAL; MARIANELA SANCHEZ; ANA RIVEROS ; DIEGO YA´NEZ; PABLO CAVALLO; MARCELO J. KOGAN ; DIEGO F.ACEVEDO

COLLOIDS AND SURFACES B-BIOINTERFACES

ELSEVIER SCIENCE BV

Lugar: Amsterdam; Año: 2016 vol. 145 p. 634 - 642

0927-7765

Inorganic materials contain remarkable properties for drug delivery, such as a large surfacearea and nanoporous structure. Among these materials, CaCO3 microparticles (CMPs) exhibita high encapsulation efficiency and solubility in acidic media. The extracellular pH of tumorneoplastic tissue is significantly lower than the extracellular pH of normal tissue facilitatingthe release of drug-encapsulating CMPs in this area. Conducting polyaniline (PANI) absorbslight energy and transforms it into localized heat to produce cell death. This work aimed togenerate hybrid CMPs loaded with PANI for photothermal therapy (PTT). The hybridnanomaterial was synthesized with CaCO3 and carboxymethyl cellulose in a simple,reproducible manner. The CMP-PANI-Cys particles were developed for the first time andrepresent a novel type of hybrid biomaterial. Resultant nanoparticles were characterizedutilizing scanning electron microscopy, dynamic light scattering, zeta potential, UV-Vis,FTIR and Raman spectroscopy. In vitro HeLa cells in dark and irradiated conditions showedthat CMP-PANI-Cys and PANI-Cys are nontoxic at the assayed concentrations. Hybridbiomaterials displayed high efficiency for potential PTT compared with PANI-Cys. Insummary, hierarchical hybrid biomaterials composed of CMPs and PANI-Cys combined withnear infrared irradiation represents a useful alternative in PTT