Synergistic effect of polyaniline coverage and surface microstructure on the inhibition of Pseudomonas aeruginosa biofilm formation

L.A. GALLARATO; L.E. MULKO; M.S. DARDANELLI; C.A. BARBERO; D.F. ACEVEDO; YSLAS * E.I.

COLLOIDS AND SURFACES B-BIOINTERFACES

ELSEVIER SCIENCE BV

Lugar: Amsterdam; Año: 2017 vol. 150 p. 1 - 7

0927-7765

Biofilm Formation is a survival strategy for microorganisms to adapt to their environment. Microbial cellsin biofilm become tolerant and resistant to antibiotics and immune responses, increasing the difficultiesfor the clinical treatment of microbial infections. The surface chemistry and the micro/nano-topographyof solid interfaces play a major role in mediating microorganism activity and adhesion. The effect of thesurface chemical composition and topography on the adhesion and viability of Pseudomonas aeruginosawas studied. Polymeric (polyethylene terephthalate) surfaces were covered with a conducting polymer(polyaniline, PANI) film by in-situ polymerization and microstructured by Direct Laser Interference Patterning(DLIP). The viability of Pseudomonas aeruginosa on the different surfaces was investigated. Thephysicochemical properties of the surfaces were characterized by water contact angle measurements,scanning electron microscopy and atomic force microscopy. Bacterial biofilms were imaged by atomicforce and scanning electron microscopies. The bacterial viability decreased on PANI compared with thesubstrate (polyethylene terephthalate) and it decreased even more upon micro-structuring the PANIfilms. In addition, the biofilm reduction could be improved using polymers with different chemical compositionand/or the same polymer with different topographies. Both methods presented diminish thebacterial attachment and biofilm formation. These findings present a high impact related to materials forbiomedical engineer applications regarding medical devices, as prostheses or catheters