Evaluation of Toxicity of nanofibers polyaniline in larvae of Rhinella (Bufo) arenarum for its application in photo-assisted therapy

YSLAS I.; IBARRA L.; TICLE M.; PERALTA D.; BARBERO C.; RIVAROLA V.; BERTUZZI M.

BIOCELL

INST HISTOL EMBRIOL-CONICET

Lugar: Córdoba; Año: 2009 vol. 23 p. 251 - 251

0327-9545

EVALUATION OF TOXICITY OF NANOFIBERS POLYANILINE IN LARVAE OF Rhinella (Bufo) arenarum FOR ITS APPLICATION IN PHOTO-ASSISTED THERAPY Yslas EI1, Ibarra L1, Ticle M1, Peralta D2, Barbero C2, Rivarola VA1, Bertuzzi M1. 1Dpto Biología Molecular, Facultad de Ciencias Exactas Físico Químicas y Naturales U.N.R.C. 2Dpto Química, Facultad de Ciencias Exactas Físico Químicas y Naturales U.N.R.C. E-mail: mbertuzzi@exa.unrc.edu.ar The photothermal therapy (PTT) is based on the irradiation of certain nanomaterials with pulses of near infrared light, which absorb light and released as heat, causing the regression tumor. The objectives of this study were: Develop the polyaniline nanostructures and dispersed, by assessing the toxicity test in Rhinella (Bufo) arenarum larvae to determine median lethal concentration, internalization and excretion. The nanofibers were synthesized by interfacial polymerization. At physiological pH the nanofibers precipitate thus solubilized in the polyvinylpyrrolidone. The toxicity studies indicated that at concentrations between 0-400 mg / L did not cause lethal damage in larvae. Moreover, it was the internalization of the drug through uv-visible absorbance and IR. Also there were observed differences in excrement´s coloration between larvae incubated with nanofibers with respect to their controls, indicating its transit through the digestive system. The nanofibers are interesting because they are biocompatible nanomaterial, uptake and no signs of toxicity in larvae. It therefore might be suitable for application in the PTT.