Photoinactivation Induced By Znpcoch3 Of Carcinoma Cell (Hep-2).

YSLAS EDITH INÉS; DURANTINI, E.N.; RIVAROLA V

BIOCELL

Inca Editorial Talleres Gráficos Cooperativa De Trabajo Ltda. Argentina

Año: 2004 vol. 24 p. 174 - 174

0327-9545

Photodynamic therapy is an alternative therapy for treatment of solid and superficial tumors and is being investigated for the treatment of arteriosclerosis, restenosis, and psoriasis. This therapy is based on the administration of a photosensitizer, which is selective retained by neoplastic tissues, and then subsequently activated with visible light of an appropriate wavelength. This new therapy results in a sequence of photochemical and photobiologic processes that cause irreversible photodamage to neoplastic tissues. This work describes the photobiologic properties of tetramethoxy Zn (II) phthalocyanine (ZnPcOCH3) on larynges carcinoma human cell line (Hep-2). The uptake of ZnPcOCH3 was linearly increasing with time for the first 1-2 h of incubation and tends to a saturation value after long incubation ≥ 3 h. Cell toxicology induced by phthalocyanine was first analyzed in dark condition and no toxicity in terms of cell survival was detected at 5 x 10-7 M. In contrast the combination of photosensitizer with visible light induced a decrease in cell survival of 20%. The morphologic analysis of the cell preformed with Hoechst-33258 revealed that the cell death after irradiation for 15 min produced chromatin fragmentation, characteristic of apoptosis.