Phosphorous deficiency in soybean plants

GALATRO A; RAMOS ARTUSO F; LIMA A; BATTHYÁNY DIGHIERO C; SANTA MARÍA G; SIMONTACCHI M

Mar del Plata

Congreso; LI Reunión Anual Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; 2015

Sociedad Argentina de Investigación en Bioquímica y Biología Molecular

Phosphorus (P) is a macronutrient necessary for energy storage and an integral component of several compounds in plant cells. Limitation of crop productivity by P is widespread and is likely to increase in the coming years. Plants have evolved different mechanisms to cope with low soil P-availability such as modification of root architecture, and exudation of organic acids. Nitric oxide (NO) plays important roles in plants, being involved in physiological processes and stress responses. The hypothesis of this work was that NO participates in early soybean responses to P restriction. Seven-day-old plants were exposed for 24 h to a medium lacking P. Total P concentration decreased significantly in roots (6.6 ± 0.6 and 3.5 ± 0.8 µmol/g FW in P+ and P-, respectively) and leaves (10 ± 1 and 5.8 ± 0.2 µmol/g FW in P+ and P-, respectively).Electron paramagnetic resonance (EPR) and fluorescence microscopy were employed to assess endogenous NO levels. Confocal laser microscopy (employing DAF-FM DA) clearly showed higher NO level in P- leaves. Analysis of nitrotyrosines by two-dimensional PAGE/western blot/Mass spectrometry showed an increase in protein tyrosine nitration in leaves of P- plants. In addition, differential leaf proteins expression was analyzed by nLC-ion trap MS. These results suggest that NO is involved in the early events related to P scarcity.PICT 2012-0429