INVESTIGADORES
MIQUET Johanna Gabriela
artículos
Título:
Upregulation of the angiotensin-converting enzyme 2/angiotensin-(1-7)/Mas receptor axis in the heart and the kidney of growth hormone receptor knock-out mice.
Autor/es:
GIANI JF; MIQUET JG; MUÑOZ MC; BURGHI V; TOBLLI JE; MASTERNAK MM; KOPCHICK JJ; BARTKE A; TURYN D; DOMINICI FP
Revista:
GROWTH HORMONE & IGF RESEARCH : OFFICIAL JOURNAL OF THE GROWTH HORMONE RESEARCH SOCIETY AND THE INTERNATIONAL IGF RESEARCH SOCIETY.
Editorial:
CHURCHILL LIVINGSTONE
Referencias:
Lugar: ESCOCIA; Año: 2012 vol. 22 p. 224 - 233
ISSN:
1096-6374
Resumen:
Objective: Growth hormone (GH) resistance leads to enhanced insulin sensitivity, decreased systolic blood
pressure and increased lifespan. The aim of this study was to determine if there is a shift in the balance of the
renin-angiotensin system (RAS) towards the ACE2/Ang-(1?7)/Mas receptor axis in the heart and the kidney
of a model of GH resistance and retarded aging, the GH receptor knockout (GHR−/−) mouse.
Growth hormone (GH) resistance leads to enhanced insulin sensitivity, decreased systolic blood
pressure and increased lifespan. The aim of this study was to determine if there is a shift in the balance of the
renin-angiotensin system (RAS) towards the ACE2/Ang-(1?7)/Mas receptor axis in the heart and the kidney
of a model of GH resistance and retarded aging, the GH receptor knockout (GHR−/−) mouse.
?7)/Mas receptor axis in the heart and the kidney
of a model of GH resistance and retarded aging, the GH receptor knockout (GHR−/−) mouse.−/−) mouse.
Design: RAS components were evaluated in the heart and the kidney of GHR−/− and control mice by
immunohistochemistry and Western blotting (n=12 for both groups).
RAS components were evaluated in the heart and the kidney of GHR−/− and control mice by
immunohistochemistry and Western blotting (n=12 for both groups).
Results: The immunostaining of Ang-(1?7) was increased in both the heart and the kidney of GHR−/− mice.
These changes were concomitant with an increased immunostaining of the Mas receptor and ACE2 in both
tissues. The immunostaining of AT1 receptor was reduced in heart and kidney of GHR−/−mice while that of
AT2 receptor was increased in the heart and unaltered in the kidney. Ang II, ACE and angiotensinogen levels
remained unaltered in the heart and the kidney of GH resistant mice. These results were confirmed by
synthase in both tissues.
Western blotting and correlated with a significant increase in the abundance of the endothelial nitric oxide
The immunostaining of Ang-(1?7) was increased in both the heart and the kidney of GHR−/− mice.
These changes were concomitant with an increased immunostaining of the Mas receptor and ACE2 in both
tissues. The immunostaining of AT1 receptor was reduced in heart and kidney of GHR−/−mice while that of
AT2 receptor was increased in the heart and unaltered in the kidney. Ang II, ACE and angiotensinogen levels
remained unaltered in the heart and the kidney of GH resistant mice. These results were confirmed by
Western blotting and correlated with a significant increase in the abundance of the endothelial nitric oxide
synthase in both tissues.
−/−mice while that of
AT2 receptor was increased in the heart and unaltered in the kidney. Ang II, ACE and angiotensinogen levels
remained unaltered in the heart and the kidney of GH resistant mice. These results were confirmed by
Western blotting and correlated with a significant increase in the abundance of the endothelial nitric oxide
synthase in both tissues.
firmed by
Western blotting and correlated with a significant increase in the abundance of the endothelial nitric oxide
synthase in both tissues.
ficant increase in the abundance of the endothelial nitric oxide
synthase in both tissues.
Conclusions: The shift within the RAS towards an exacerbation of the ACE2/Ang-(1?7)/Mas receptor axis
observed in GHR−/−mice could be related to a protective role in cardiac and renal function; and thus, possibly
contribute to the decreased incidence of cardiovascular diseases displayed by this animal model of longevity
The shift within the RAS towards an exacerbation of the ACE2/Ang-(1?7)/Mas receptor axis
observed in GHR−/−mice could be related to a protective role in cardiac and renal function; and thus, possibly
contribute to the decreased incidence of cardiovascular diseases displayed by this animal model of longevity
−/−mice could be related to a protective role in cardiac and renal function; and thus, possibly
contribute to the decreased incidence of cardiovascular diseases displayed by this animal model of longevity