cDNA Sequence Coding of the Third ComplementComponent in Broad-Snouted Caiman (Caiman latirostris)

SIROSKI, P; MARELLI, ; BELKIS; ORTEGA, H; MUDRY, M. D

Memphis

Congreso; XXIII Congress of CSG; 2014

Cocrodilians Specialists Group (CSG)

From several years ago, we are talking and reporting some important characteristics of crocodilian immune system (IS) and its ability to avoid spreading infection. Likewise, many components of this IS were reported but few studies have been go into moleculardetails. If we can detect the origin of these abilities, we could think the idea to replicate it and apply therapeutically. The complement system (SC) is one of the major effector mechanisms of the IS and it was reported in crocodilians IS. It is a set of 30 different plasma proteins that act sequentially and whose function consists in recruiting effectors of pro-inflammatory cells, opsonization and lysis of pathogens. The SC is activated by three different pathways and the initiation of any of the 3-way is performed by activation of C3, the main protein. From theC. latirostrispartial genome sequence and the alignment of the similar species sequence from National Center for Biotechnology Information (NCBI) were designed a group of random primers to identified a sequencewith high similarity to C3. Nowadays, 90 % of Caiman C3 was identified and by rapid amplification of the cDNA ends (RACE) is yielding the full open reading frame. This previous characterization is the first step to perform a first evaluation of the future application of products derivate from this species innon-specific antimicrobial therapy in animals of zootechnical interest. These findings generate an enhancement of the resource that is being subject to sustainable management programs with a major social impact.