Molecular identification of Anaplasma marginale in two autochthonous South American wild species revealed an identical new genotype and its phylogenetic relationship with those of bovines

DE LA FOURNIERE S; CORREA E; PAOLETTA M; WILKOWSKY S; GUILLEMI EC; PEÑA MARTINEZ J; LOPEZ ARIAS L; PINARELLO V; OROZCO MM; FERNANDEZ J; CORONA B; FARBER M.

PARASITES AND VECTORS

BIOMED CENTRAL LTD

Lugar: Londres; Año: 2016 vol. 9 p. 1 - 9

1756-3305

Background: Anaplasma marginale is a well known cattle pathogen of tropical and subtropical world regions. Eventhough, this obligate intracellular bacteria has been reported in other host species different than bovine, it has never been documented in Myrmecophaga tridactyla (giant anteater) or Hippocamelus antisense (taruca), which are two native endangered species.Methods: Samples from two sick wild animals: a Myrmecophaga tridactyla (blood) and a Hippocamelus antisense (blood and serum) were studied for the presence of A. marginale DNA through msp5 gene fragment amplification. Further characterization was done through MSP1a tandem repeats analysis and MLST scheme and the genetic relationship among previously characterized A. marginale sequences were studied by applying, eBURST algorithm and AMOVA analysis.Results: A. marginale DNA was identified in the Myrmecophaga tridactyla and Hippocamelus antisense samples. Through molecular markers, we identified an identical genotype in both animals that was not previously reported in cattle. Through eBURST analysis this variant was arranged next to local genotypes eventhough it was not possible to define a clear geographical association. Conclusions: In the present publication we report the identification of A. marginale DNA in a novel ruminant (Hippocamelus antisense) and non-ruminant (Myrmecophaga tridactyla) host species. Further analysis is needed to understand whether these two hosts contribute to the Anaplasmosis epidemiology.